干旱胁迫下杨树嫩茎质外体内源激素的响应

Responses of Endogenous Hormones to Drought Stress in the Apoplast of Populus

质外体与植物细胞有着不可分割的联系,其内发生的干旱胁迫响应鲜见报道,因此本文采用MD-HPLC联用技术对3种杨树嫩茎质外体内源激素在干旱胁迫胁迫下的变化进行研究。结果表明:随着干旱胁迫程度的加剧和时间的延长,3种杨树质外体GA3、6-BA和3-IAA含量明显减少,而ABA含量极显著增加且GA3、6-BA、3-IAA和ABA含量的变化品种间差异显著。该研究为植物干旱胁迫生理响应机制研究提供新依据,为活体、动态地定量分析质外体内源激素提供了新方法。

The apoplast and plant cells are inextricably linked, yet the response to drought stress within apoplast has rarely been reported. In this paper, the drought stress responses of apoplastic endogenous hormones in tender stems of three poplar species were studied by using MD-HPLC coupled technique. Four kinds of endogenous hormones in the apoplast of tender stems of living poplar which were dialyzed by microdialysis probe, i. e. benzylaminopurine （6-BA）, gibberellic acid （ GA3 ）, indole aceticacid （ 3-IAA）, and abscisic acid （ ABA ）, were then simultaneously determined by HPLC. The results show that drought stress has a significant effect on the content of endogenous hormones within the apoplast （P 〈 0.05）. As the drought stress was intensified and lasting, the contents of GA3, 6-BA and 3-IAA obviously decreased while the content of ABA increased significantly （P 〈 0.01 ） , meanwhile the contents of GA3 , 6-BA, 3-IAA and ABA has changed differently among poplar species. This paper has provided a new basis for study on physiological response mechanism to drought stress and it also provides a new method for the dynamically quantitative analysis of the apoplastic endogenous hormone in vivo.