五月季竹开花及复壮过程中DNA甲基化的MSAP分析(英文)

Analysis on DNA Methylation-sensitive Amplified Polymorphism in Phyllostachys bambusoides During Flowering and Rejuvenation

以五月季竹为材料,采用MSAP技术对其开花及花后无性复壮过程中的DNA甲基化状况进行检测,分析其开花前后的甲基化动态,以揭示竹子开花及复壮过程中的表观遗传变化规律。结果显示:(1)五月季竹开花时其叶片甲基化水平降低,而在无性复壮产生不再开花新竹的过程中其叶片甲基化水平又逐渐回升;(2)与未开花竹株相比,五月季竹开花时有29.09%的甲基化位点发生了变异,其中有17.88%的位点在开花植株中发生了完全的去甲基化,远高于发生甲基化位点的比率;(3)复壮竹株与未开花竹株之间发生变异的位点数和所占比率,尤其是发生去甲基化的位点数和比率,低于开花竹株;(4)开花五月季竹花器官的甲基化水平低于叶片,同时有28.58%的位点发生了甲基化状态的改变,且同样以去甲基化为主。

To reveal epigenetic changes during flowering and rejuvenation of bamboo, we performed methylation- sensitive amplification polymorphism （MSAP） analysis on the genomic DNA of Phyllostachys bambusoides Sieb. et Zucc. during flowering and asexual rejuvenation. Methylation level in P. bambusoides blades decreased during the flowering stage and then increased gradually after flowering when new l^amboo stands were produced via asexual rejuvenation. Compared with non-flowering bamboo stands, 29.09% of methylation sites were mutated during P. bambusoides flowering, with 17.88% of sites completely demethylated in flowering plants, which was much higher than that of the methylation sites （3.61%）. The number and ratio of polymorphic sites changing in methylation status occurred in rejuvenated and non-flowering bamboo stands, especially demethylated sites, were lower than those in flowering bamboo stands. Methylation in floral organs was lower than that in blades in flowering P. bambusoides, and 28.58% of sites demonstrated changes in methylation status, mainly in the form of demethylation.