中药提取物对耐药幽门螺杆菌生物膜形成的影响

Effect on the Biofilm Formation of Drug Resistant Helicobacter Pylori by Chinese Herbal Extracts

目的观察中药提取物对耐药幽门螺杆菌生物膜形成的影响。方法采用标准琼脂平板对倍稀释法测定大黄素、黄连素、苦参碱、黄芩苷等对耐药幽门螺杆菌的最低抑菌浓度(MIC),检测低于MIC药物作用菌株后反应孔的吸光度(A)值,并通过共聚焦显微镜观察耐药幽门螺杆菌生物膜形成情况。结果大黄素、黄连素、苦参碱、黄芩苷对耐药幽门螺杆菌的MIC分别是64,64,128,256μg·mL-1;大黄素32μg·mL-1、黄连素32μg·mL-1、苦参碱64μg·mL-1、黄芩苷128μg·mL-1时,共聚焦显微镜下生物膜形成明显较少。结论大黄素、黄连素、苦参碱、黄芩苷对耐药幽门螺杆菌有抑制作用,浓度<50%MIC能明显抑制菌株生物膜形成。

Objective To explore the influence of Chinese herbal extracts on biofilm formation of drug resistant Helicobacter pylori. Methods The standard agar dilution method was used to determine the MIC of emodin, berberine, matrine, and baiealin to drug resistant Helicobacter pylori, and detect the optical density under concentrations less than MIC. Confocal microscopy was applied to observe biofilm formation of drug resistant Helicobacter pylori. Results The MIC of emodin, berherine,matrine and baicaliu on drug resistant Helicobacter pylori were 64,64,128 ,256 ixg . mL^-1, respectively. Biofilm formation of Helicobacter pylori was significantly less under confocal microscope with the concentrations of emodin, berberine, matrine and baicalin at 32 μg .mL^-1 ,32 μg .mL^-1 ,64 μg . mL^-1 and 128μg . mL^-1 ,respectively. Conclusion Emodin, berberine, matrine and baicalin can inhibit drug resistance Helieobacter pylori, and suppress the formation of bacteria biofilm at the dose of 50% MIC.