荞麦花叶提取物对荷瘤小鼠免疫功能的影响

Effect of the Extraction of Buckwheat Flower and Leaf on Immunity Function of H22 Tumor Mice

目的观察荞麦花叶提取物(EBFL)对H22荷瘤小鼠免疫功能的影响,探讨EBFL辅助抗肿瘤作用机制。方法复制小鼠H22实体瘤模型,随机分为EBFL低(0.2 g·kg-1·d-1)、高剂量组(0.4 g·kg-1·d-1)和环磷酰胺(CTX)组(0.025 g·kg-1)、EBFL高剂量(0.4 g·kg-1·d-1)+CTX组(0.025 g·kg-1)、模型组(0.9%氯化钠溶液),设正常小鼠为对照组。EBFL和0.9%氯化钠溶液灌胃给药,连续14 d;CTX腹腔注射,隔日1次。停药次日称瘤质量,检测血常规。碳廓清法检测吞噬细胞功能,噻唑蓝法观察小鼠脾淋巴细胞转化功能。结果 EBFL对荷瘤小鼠肿瘤生长有一定抑制作用,但不明显,与CTX组比较差异有统计学意义(P<0.05)。EBFL能增加荷瘤小鼠巨噬细胞吞噬指数和淋巴细胞转化率,EBFL高剂量组吞噬指数(0.079±0.020),淋巴细胞转化率(0.298±0.033),与模型组、CTX组比较,均差异有统计学意义(P<0.01)。结论 EBFL可提高H22荷瘤小鼠免疫功能,减轻CTX所致小鼠免疫抑制。

Objective To investigate the effect of EBFL on the immune function of H22 tumor mice and explore its possible anti-tumor mechanism. Methods The abdominal cavity of mice was inoculated with H22 cells to maked ascites tumor model. The mice were divided into EBFL low dose（0. 2 g . kg^-1 .d^-1）,EBFL high dose（0. 4 g. kg^-1 . d^-1）,CTX （0.025 g . kg^-1） ,EBFL high（0.4 g ~ kg-1 .d^-1 ）plus CTX （0.025 g . kg^-1 ）, model and control groups. EBFL was administered intragastrieally every day for 2 weeks and CTX was given as intraperitoneal injection every other day. Routine blood examination was performed. The thymus gland and spleen were weighed, and thymus gland （spleen） index after administration for 2 weeks was calculated. Phagocyte function was examined by carbon particle clearance method and the cell conversion function of spleen lymphoid cells was observed with MTr method. Results EBFL slightly inhibited growth of I-I22 tumor in mice, but tumor inhibition effect of EBFL was significantly less than that of CTX（ P〈O. 05 ）. Phagocytosis index of phagocytes （0.079＋0.020）and lymphoid cell conversion rate （0. 298±0. 033 ）in EBFL high group were significantly higher than those in model group and CTX group. Conclusion EBFL could alleviate CTX-induced immunosupression by boosting the immune function of H22 tumor mice.