逍遥散对慢性应激大鼠LC/NE系统的干预效应

Intervention Effect of Ease Powder on Locus Coeruleus /Noradrenergic System of Rats with Chronic Unpredictable Stress

目的观察慢性应激状态下大鼠行为学改变及蓝斑(LC)内酪氨酸羟化酶(TH)和多巴胺-β-羟化酶(DBH)的基因表达,以及逍遥散的治疗作用。方法将24只SD健康雄性大鼠随机分为对照组、模型组、逍遥散治疗组和氟西汀治疗组(n=6),采用束缚方法造模,观察大鼠行为学改变。造模后第22天取出大鼠脑干LC,实时荧光定量聚合酶链反应(PCR)检测LC中TH mRNA、DBH mRNA表达。结果与对照组比较,模型组穿越格数、直立次数和理毛次数均显著减少,差异有统计学意义(P<0.01);造模后LC中TH mRNA、DBH mRNA表达均显著升高,差异有统计学意义(P<0.01);逍遥散治疗组和氟西汀治疗组与模型组比较,大鼠穿越格数、直立次数和理毛次数显著升高,差异有统计学意义(P<0.01);与对照组比较,逍遥散治疗组、氟西汀治疗组大鼠穿越格数、直立次数、理毛次数,以及LC中TH mRNA、DBH mRNA表达均差异无统计学意义。结论慢性应激激活了LC-去甲肾上腺素能系统,使其释放儿茶酚胺类神经递质去甲肾上腺素,其合成酶TH、DBH表达增高,进而调控机体兴奋、紧张、焦虑等情绪反应。逍遥散可有效干预,下调其过表达。

Objective To study the abnormal behavior and expression of tyrosine hydroxylas （TH） and dopamine-β- hydroxylase （DBH） in Locus coeruleus （LC） in rats under chronic unpredictable stress, and evaluate the therapeutic effect of Ease powder. Methods A total of 24 male SD rats were randomly divided into control, model, and treatment （ease powder, fluoxetine） groups （n = 6 in each group）. The rat model was established by body tight-binding. The open-field test was performed. The LC was isolated from the brains 22 days post modeling, and the mRNA expression levels of TH and DBH in LC were analyzed by real-time PCR. Results Compared with normal control, the squares crossed, rearing and grooming were significantly reduced （P〈0.01） ,and the mRNA expression levels of TH and DBH were significantly increased （P〈0.01） in the model group. As compared to the model control, the ease powder- and fluoxetine-treated groups exhibited significant increase in the squares crossed,rearing, grooming time and decrease in mRNA expression levels of TH and DBH （P〈0.01） . Conclusion Chronic unpredictable stress regulates body＇s mood reactionlike excitement, stress and anxiety by activating the LC/NE （ Locus coeruleus/noradrenergic） system,leading to catecholamine release and TH and DBH increase in NE neurons. The ease powder can effectively reverse the abnormal behavior and down-regulate TH and DBH expression.