佛手散水煎剂高效液相色谱指纹图谱研究

HPLC Fingerprint of Fushousan Decoction

目的建立佛手散水煎剂高效液相色谱指纹图谱，为该方的物质基础和质量控制提供科学依据。方法Atlantis仍柱（4．6mm×250mm，5μm），流动相为甲醇-0．2％醋酸水溶液；梯度洗脱，流速0．8mL·min^-1，检测波长290nm，柱温25℃。以阿魏酸为参照物，在相同的色谱条件下测定10批佛手散水煎剂样品。结果建立了佛手散水煎剂的高效液相色谱指纹图谱，标定了16个共有指纹峰，其中12个同时来自当归和川芎，4个来自川芎。10批样品的相似度在0．943—0．989之间。结论建立的方法简便、准确、重复性好，为佛手散水煎剂及相关制剂的的质量控制提供科学依据，为佛手散水煎剂药效物质基础研究提供参考。

Objective To establish the HPLC fingerprint offushousan decoction. Methods A HPLC method was established with Atlantis T3 column （4.6 mm×250 mm,5 μm） , using methanol-water-0.2% acetic acid as the mobile phase, setting the flow rate at 0.8 mL . min^-1 and the detection wavelength at 290 nm. By taking ferulic acid as the reference substance, the fingerprints of 10 batches offushousan decoction were analyzed under the same chromotographic condition. Results The HPLC fingerprints offushousan decotion were established, and sixteen common peaks were signed, in which four were originated from Rhizoma chuanxiong and twelve were from both Radix Angelica sinensis and Rhizoma chuanxiong. The similarity threshold of ten batches of samples was between 0. 943-0.989. Conclusion This method is accurate and reproducible, providing a certain reference to effective substances study and quality control offushousan.