鄂产竹节参高效液相特征图谱的建立和分析

Studies on the Specific Chromatogram of Panax Japonicus in Hubei by HPLC

目的建立鄂产竹节参药材的高效液相色谱法指纹图谱。方法采用Apollo C18(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速1 mL·min-1,检测波长203 nm,柱温:30℃。结果测试样品10批,以竹节参皂苷Ⅴ色谱峰为参照峰,确立4个共有峰作为竹节参药材特征峰,测得其含量分别为12.616,3.976,5.625,4.429 mg·g-1。结论竹节参药材特征图谱专属性强,可用于鄂产竹节参药材的鉴定及质量控制。

Objective To establish the HPLC finger-print for Panax japonicus produced in Hubei. Methods The analysis was performed on a Apollo Cls column（4.6 mm×250 mm,5 μm） ,using the mobile phase of acetonitrile-0.05% solution phosphoric acid; the detection wavelength was 203 nm; the column temperature was 30 ℃ and the flow rate was 1 mL . min^-1. Results Ten samples collected in different picking time common peaks were confirmed in specific chromatograms. Four compounds were elucidated with the peak of Chikusetsu saponin V as reference. The content of 4 major common peaks were 12. 616,3. 976,5. 625, and 4. 429 mg . g^-1, respectively. Conclusion The method can be applied to the quality control of Panax Japonicum C. A. Mey. in Hubei.