南荻MlNAC13基因功能的初步探究

Preliminary functional analysis of MlNAC13 gene in Miscanthus lutarioriparius

对南荻MINAC13基因进行了克隆、亚细胞定位和转录激活试验,并用实时荧光定量PCR方法,检测了在盐胁迫、干旱胁迫、低温和脱落酸、茉莉酸甲酯、水杨酸处理下的MlNAC13基因在南荻根部的表达模式。结果:1)获得了南荻MINAC13的全基因序列;2)用N端融合YFP进行亚细胞定位试验的结果证明,MlNAC13位于细胞核,MlNAC13可能在细胞核内行使功能;3)MlNAC13的C端及全长具有转录激活活性,N端没有转录激活活性,说明它是转录因子,且转录激活域位于C端;4)除水杨酸外,脱落酸、茉莉酸甲酯均能诱导MlNAC13基因的表达,说明MlNAC13基因可能参与南荻的多种逆境胁迫响应。

MlNAC13 gene was cloned from Miscanthus lutarioriparius （M. lutarioriparius）, and subcellular localization and transactivation assay were conducted on this gene. Then real-time quantitative PCR （RT–qPCR） was used to analyze the expression profile of MlNAC13 in root under salt, drought, cold, abscisic acid （ABA）, Methyl jasmonate （MeJA） and Salicylic acid （SA） treatments. The results showed that 1） complete MlNAC13 gene sequence of M. lutaroriparius was obtained; 2） subcellular localization analysis using N-terminus fused with YFP showed that MlNAC13 was located in nucleus, suggesting that it might function in nucleus; 3） transactivation assay indicated that the full-length and C-terminus of MlNAC13 had transactivation activity, indicating that MlNAC13 is a transcription factor and the transactivation activity region located in C-terminus; 4） the transcript of MlNAC13 was up-regulated under all surveyed treatments except SA, indicating that it might participate in various stress responses of M. lutarioriparius.