拟南芥含MATH结构域基因AtSb3的表达分析

Analysis of the expression of AtSb3 gene containing MATH domains in Arabidopsis thaliana

利用RT–PCR的方法检测了AtSb3在拟南芥的表达情况;在构建AtSb3基因启动子与GUS融合表达载体并获得拟南芥转化子的基础上,用组织化学染色方法对AtSb3基因在拟南芥中的表达进行了研究。RT–PCR的结果表明:只在拟南芥根中检测到AtSb3的表达,在茎、叶、花和果荚中均检测不到AtSb3的表达。GUS组织化学染色结果显示:在刚萌动的种子中,AtSb3主要在胚根部位表达,其他部位不表达;在种子萌发后的子叶展开期,AtSb3在根尖部位表达,其他部位不表达;在4～5叶期幼苗中,AtSb3在地上部分没有表达,只在主根和各级侧根的根尖部位表达;在根尖部位,AtSb3基因在根冠与分生区细胞结合部位,以及伸长区和根毛区的表达量高,而在分生区与伸长区之间存在一段不表达的区域。

RT-PCR were used to investigate the expression of Atsb3 in Arabidopsis thaliana, and the vector with AtSb3 gene promoter and GUS fusion expression cassette was constructed to obtain the transgenic lines ofArabidopsis thaliana, based on which the tissue localization ofAtSb3 gene expression was investigated by histochemical staining. The results of RT-PCR showed AtSb3 gene was expressed only in root, not in the stem, leaf, flower and silique. The results of GUS staining showed that in seeds at early germinating stage, AtSb3 gene was only expressed in radicle. In cotyledon expansion period after seed germination, AtSb3 gene expression was only detected in root tip, not in other parts of the plant. In 4 to 5 leaf stage seedling, AtSb3 was not expressed in aboveground parts but in taproots and lateral roots. In the root tip, GUS staining was heavier in conjunction region of the root cap and meristematic zone, as well as in elongation zone and root hair, which indicated expression level ofAtSb3 gene was higher in these parts; GUS staining in the region between meristematic zone and elongation zone was not observed.