丹参酮亚微Ⅱ_A亚乳剂的制备及其HPLC法测定

Preparation of Perillyl Alcohol Submicron Emulsions and Content Determination by HPLC

目的 制备了丹参酮ⅡA亚微乳,进行HPLC法测定.方法 两步高压乳匀法制备丹参酮ⅡA亚微乳,使用c18柱,以乙腈：水（80：20）为流动相,检测波长270nm,建立HPLC法测定丹参酮ⅡA亚微乳的含量.结果 研制的亚微乳平均粒径为（191.3±0.56）nm,Zeta电位为（41.76±0.31） mV.丹参酮ⅡA在0.25～4mg/ml浓度范围内存在良好的线性关系（r=0.9999）,三个浓度样品的回收率分别为（99.74±0.78）、（100.53±1.25）、（99.11±0.60）,日内和日间RSD分别为1.76%、0.79%、0.75%和1.93%、1.50%、0.68%.结论

Objective To determine the content of tanshinone lI A submicron emulsion by HPLC. Methods The 2 steps of high pressure homogenization preparation of tanshinone Ⅱ A submicron emulsion was using C18 column, acetonitrile： water （80： 20） as the mobile phase. The detection wavelength was 270nm. The content determination of tanshinone Ⅱ A submicron emulsion would be established by HPLC method. Results The average particle size of submicron emulsion was （ 191.3 ± 0. 56） nm, Zeta potential was （41.76 ± 0. 31 ） inV. Tanshinone II A in 0. 25 - 4mg＇ ml concentration range of memory in a good linear relationship （ r = 0. 9999） , recovery of the three samples were （99.74 ± 0.78） , （100. 53± 1.25） , （99. 11 ±0.60） , the intra-day and inter-day RSD were 1.76% , 0.79% , 0. 75% and 1.93% , 1.50% , 0. 68%. Conclusion Tanshinone Ⅱ A submieron emulsion was stable.