女性不孕不育患者血清及宫颈黏液中相关抗体检测的实验研究

An experimental study on the detection of related antibodies in serum and cervical mucus of patients with infertility

目的:探讨女性不孕不育患者血清及宫颈粘液中抗精子抗体(AsAb)、抗子宫内膜抗体(EmAb)、抗心磷脂抗体(ACAb)和抗卵巢抗体(AoAb)的临床检测意义。方法:选健康女性体检者20例作为对照组,不孕不育女性80例作为实验组,采用ELISA酶联免疫法检测血清及宫颈粘液中AsAb、EmAb、ACAb和AoAb的蛋白表达水平。结果:与对照组相比较,实验组血清中AsAb、EmAb、ACAb和AoAb的蛋白表达水平均明显升高,差异有统计学意义(P<0.01);宫颈粘液中AsAb、EmAb和ACAb的蛋白表达水平升高更为明显,差异有统计学意义(P<0.01),AoAb的蛋白表达水平差异无统计学意义(P>0.05)。结论:AsAb、EmAb、ACAb和AoAb与不孕不育关系密切,其检测有助于免疫性不孕不育症的诊断。

Objective： To explore the clinical significances of detection of anti - sperm antibody （ AsAb）, anti - endometrium anti- body （ EmAb）, anti - cardiolipin antibody （ ACAb）, and anti - ovary antibody （AoAb） in serum and cervical mucus of patients with infer- tility. Methods： Twenty women receiving healthy physical examination in the hospital were selected as control group, and eighty infertile women were selected as experimental group; ELISA was used to detect the expression levels of AsAb, EmAb, ACAb, and AoAb proteins in serum and cervical mucus. Results： Compared with control group, the expression levels of AsAb, EmAb, ACAb, and AoAb proteins in serum of experimental group increased significantly, there were statistically significant differences （ P 〈 0. 01 ） ; and the increases of expres- sion levels of AsAb, EmAb, and ACAb proteins in cervical mucus were more obvious, there were statistically significant differences （P 〈 0. 01 ）, there was no statistically significant difference in the expression level of AoAb protein （P 〉 0. 05 ） . Conclusion： AsAb, EmAb, ACAb, and AoAb are closely correlated with infertility, the detection of them is helpful to diagnose immune infertility.