四倍体刺槐黄化嫩枝扦插生根过程生理生化分析

Physiology and Biochemistry during Etiolated Shoots Cutting of Tetraploid Robinia pseudoacacia

以四倍体刺槐1年生硬枝进行黄化催芽,获得的黄化和未黄化嫩枝为扦插材料,统计和比较了二者的生根情况,用比色法和间接酶联免疫法分别对扦插后不同时间的材料进行了吲哚乙酸氧化酶、多酚氧化酶和过氧化物酶3种氧化酶的活性测定和吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素和玉米素核苷4种内源激素的质量分数测定,通过对比两种材料在扦插后的不同时期的氧化酶活性和4种内源激素的质量分数的差异及变化规律。结果表明:黄化插穗在生根率、平均生根数和平均根长度方面均明显优于未黄化插穗,并且2种处理方式的3种氧化酶活性和4种内源激素质量分数的变化趋势大致相同,但是黄化嫩枝含有更多的能够促进不定根形成的内源激素,并且氧化酶的活性以及IAA/ABA值都较高,表明黄化处理后的插穗通过氧化酶和内源激素的变化调节,进而促进不定根形成。

The experiment was conducted to study the contrast of the abilities of rooting during the cuttage between etiolated and non-etiolated Tetraploid Robinia pseudoacacia shoots. Colorimetry and indirect enzyme-linked immunosorbent assay （ ELI- As） were used to detect the level of three kinds of oxidase including indole acetic acid oxidase （IAAO）, polyphenol oxi- dase enzyme （PPO） and peroxidase （POD）, and four kinds of endogenous hormone including indole acetic acid （IAA）, abscisic acid （ ABA）, gibberellic acid （ GA3 ） and zeatin riboside （ZR） in Tetraploid Robinia pseudoacacia etiolated shoots and non-etiolated shoots after the cuttage. Through the contrast of the differences and variation of oxidase activity and endogenous hormones content in the two kinds of materials, etiolated shoots are significantly superior on rooting rate, average root number and average root length . Three kinds of oxidase activities and four kinds of endogenous hormones un- der two treatments roughly have the same variation trends , but the etiolated shoots can form more IAA to promote the for- mation of roots, and have a higher levels of oxidase enzyme activity as well as IAA/ABA values. Therefore, the etiolated shoots can improve the oxidase activities, regulate endogenous hormones and the formation of adventitious roots.