α-三噻吩对松材线虫的室内毒力测定

Nematicidal Toxicity Test of α-Terthienyl against Pine Wood Nematodes (Bursaphelenchus xylophilus) in Laboratory

研究了黑松(Pinus thunbergii)木屑不同含水量对松材线虫(Bursaphelenchus xylophilus)繁殖的影响,确定了适于线虫繁殖的最佳含水量,并以黑松木屑作为试验介质,模拟松材线虫生活条件,测定了杀线剂α-三噻吩(α-T)对松材线虫的毒力。结果表明:当黑松木屑相对含水量达到50%时最有利于松材线虫繁殖;α-T对松材线虫具有非常强的毒性,毒力回归方程为Y=14.589+18.720x,半致死质量分数为1.892μg/g。

An experiment was conducted to measure nematicidal activity of pine wood nematodes （Bursaphelenchus xylophilus ） with sawdust of black pine （Pinus thunbergii） , and determine the best relative water content （RWC） of the sawdust to culture pine wood nematodes and bioassay the nematicidal activity of α-T by the method. 50% of RWC is best suitable condition to culture pine wood nematodes, and by the regression equation of α-T against pine wood nematodes under the light intensity of 10 000 Lux, the toxicity regression equation is Y= 14. 589＋18. 720x, and the medial lethal concentration is LC50 = 1. 892 μg/g. Keywords α-Terthienyl ; Bursaphelenchus xylophilus ; Toxicity regression