还阳参多糖CTP3-E的结构分析

Structural Characterization of the Polysaccharide CTP3-E from Crepis turczaniowii C. A. Mey.

目的研究还阳参(Crepis turczaninowii C.A.Mey)中分离纯化的纯多糖CTP3-E结构分析。方法采用糖组成分析、甲基化分析和波谱分析方法确定CTP3-E的结构。结果 CTP3-E重均分子量为12 182,由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成。红外光谱和核磁共振波谱(Dept,HMBC,HMQC等)研究确定CTP3-E化学结构。结论CTP3-E是首次从该植物中分离得到的酸性杂多糖。

OBJECTIVE To characterize the chemical structure of a homogenous polysaccharide CTP3-E purified from Crepis turc- zaninowii C. A. Mey. METHODS Sugar composition analysis, methylation analysis, IR and NMR （Dept, HMBC, HMQC, etc. ） were used to elucidate the structure. RESULTS The molecular weight of CTP3-E was 12182. CTP3-E was composed of mannose, rhamose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. CONCLUSION CTP3-E is a new acidic hetero- polysaccharide and was isolated from this medicinal plant for the first time.