知母降糖有效部位的提取工艺优选及成分分析

Optimization of Extraction Process and Component Analysis of Hypoglycemic Effective Parts from Anemarrhenae Rhizoma

目的:优选知母中降糖有效部位的提取工艺并鉴定其成分。方法:以总皂苷得率及浸膏得率为指标,通过均匀设计试验考察乙醇体积分数、乙醇用量及提取时间对工艺的影响;以双苯吡酮类成分、总皂苷提取率为指标,利用单因素试验筛选提取次数。采用紫外分光光度法测定双苯吡酮类成分含量,HPLC测定总皂苷含量,利用UPLC/Q-TOF-MS技术分析知母降糖有效部位的主要成分。结果:知母降糖有效部位的最佳提取工艺为加8倍量80%乙醇提取2次,每次1 h,其主要成分包括43种知母皂苷类和7种双苯吡酮类成分。结论:优选的提取工艺稳定可行,为糖尿病及其并发症的中药有效部位药物研发提供参考。

Objective： To optimize extraction process of hypoglycemic effective parts from Anemarrhenae Rhizoma and identify its ingredients. Method： With yield of total saponins and extract yield as indexes, uniform design test was adopted to investigate effects of ethanol concentration, ethanol amount and extraction on technology; With yield of total saponins and xanthones as indexes, extraction times was screened by single factor test. The content of xanthones was determined by UV, while total saponins was determined by HPLC, main components of hypoglycemic effective parts from Anemarrhenae Rhizoma were identified by UPLC/Q-TOF-MS. Result： Optimal extracting technology was as following： extracted twice with eight times the amount of 80% ethanol for 1 h per time; Main components of hypoglycemic effective parts from Anemarrhenae Rhizoma included 43 timosaponins and 7 xanthones. Conclusion： This optimized technology was stable and feasibel, it provided a reference for drug developing of effective parts from Chinese materia medica, which were adopted to treat diabetes and its complications.