响应面法优化阳春砂多糖的超声辅助提取工艺

Optimization of Ultrasound-Assisted Extraction Technology of Polysaccharides from Amomum villosum by Response Surface Methodology

目的:优选阳春砂多糖的超声提取工艺条件。方法:以阳春砂多糖提取率为评价指标,在单因素试验基础上,选定提取温度、时间及料液比为考察因素,通过三因素三水平Box-Behnken中心组合试验建立多糖提取率的二次多项式回归方程,经响应面回归分析得到优化组合条件。采用紫外分光光度法测定多糖含量。结果:最佳提取工艺条件为提取温度78℃,超声时间71 min,料液比1∶39,多糖提取率实测值(13.04±0.36)%,与预测值(13.22%)较为接近。结论:与传统水提取法相比,超声辅助法具有省时、高效的优点,为阳春砂多糖的后续产品研发提供实验依据。

Objective： To optimize ultrasound-assisted extraction technology of polysaccharides from Amomum villosum by response surface analysis. Method： On the basis of single-factor tests, with yield of polysaccharides from A. villosurn as index, quadratic polynomial regression equation of polysaccharides yield was established by a three-factor and three-level Box-Behnken Design with solid-liquid ratio, extraction time and temperature as factors, then the established model was analyzed by response surface methodology to obtain optimum extraction process. The content of polysaccharides was determined by UV. Result： Optimum extraction conditions were as follows： extracting time 71 min, solid-liquid ratio 39：1 and extraction temperature 78 ~C. Under these conditions, the estimated and observed values of yield of polysaceharides were 13.22% and （13.04 -＋0. 36）% , respectively. Conclusion： Compared with traditional water extraction, ultrasound-assisted method had advantages of time-saving and efficient, this study could provide experimental basis for the follow-up research and development of polysaccharides in A. villosum.