不同采收期、加工方法对溪黄草中咖啡酸和迷迭香酸含量的影响

Effects of Different Harvest Times and Different Processing Methods on Contents of Caffeic Acid and Rosmarinic Acid in Isodon lophanthoides

目的：考察不同采收期和加工方法对溪黄草中咖啡酸、迷迭香酸含量的影响.方法：采用HPLC测定咖啡酸、迷迭香酸含量,色谱条件为Dikma Diamonsil C18（2）色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈（A）-0.3％磷酸溶液（B）梯度洗脱（0 ～ 17 min,12％A;17～20 min,12％ ～25％ A;20 ～50 min,25％A）,流速1.0 mL· min-1,检测波长329 nm,柱温室温,进样量20 μL.以咖啡酸、迷迭香酸含量为指标,考察采收期（5～11月份）和干燥方法（烘干、晒干和阴干）对溪黄草药材质量的影响.结果：不同采收期对溪黄草茎、叶中咖啡酸含量影响较小,但对迷迭香酸含量的影响较大.3种干燥方法处理后迷迭香酸提取率分别为1.365％,1.790％,1.577％,咖啡酸提取率分别为0.057％,0.053％,0.055％.结论：以7月份采收的药材质量最佳,可每年采收2次,采收时间为7月和11月;药材加工方法采用自然晒干为最佳.

Objective： To investigated effects of different harvesting times and different processing methods on the contents of caffeic acid and rosmarinie acid from Isodon lophanthoides. Method： The contents of eaffeic acid and rosmarinic acid were determined by HPLC, which was performed on a Dikma Diamonsil Cls （2） column （4.6 mm x250 mm, 5 ~xm）, gradient elution （0-17 min, 12%A; 17-20 min, 12%-25%A; 20-50 min, 25%A） with mobile phase of acetonitrile-0.3% phosphoric acid, flow rate was 1.0 mL .min-1, column temperature was room temperature, detection wavelength was 329 nm, injection volume 20 ixL. With the contents of caffeic acid and rosmarinic acid as indexes, effects of harvesting times （May to November） and processing methods （drying in shade, sun-dried, drying） on quality of L lophanthoides were investigated. Result： Different harvesting times had little effect on the content of caffeie acid from stems and leaves of L lophanthoides, but it had significant effect on the content of rosmarinic acid. After handled by three drying methods, yield of rosmarinic acid were 1. 365% 1. 790% , 1. 577% , respectively; Yield of caffeic acid were 0. 057% , 0. 053% , 0. 055% , respectively. Conclusion： L Lophanthoides harvested in July had the best quality, herbs could be harvested twice a year, harvesting times were in July and November; Processing method of naturally dried was optimum.