狭基线纹香茶菜(溪黄草)咖啡酸和迷迭香酸的薄层鉴别与含量测定

Identification and Determination of Caffeic Acid and Rosmarinic Acid in Rabdosia lophanthoides var gerardiana by TLC and HPLC

目的：建立狭基线纹香茶菜药材中咖啡酸和迷迭香酸的TLC鉴别及HPLC含量测定方法.方法：以正已烷-乙酸乙酯-甲酸（3：3：1）为展开剂,展开后取出晾干,于干燥器内放置过夜后于紫外灯（365 nm）下检视;Dikma Diamonsil C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.3％磷酸,流速为1.0 mL·min-1,检测波长为329 nm.结果：在TLC色谱中咖啡酸和迷迭香酸斑点清晰,分离效果好;咖啡酸和迷迭香酸分别在0.045 4 ～0.908 0μg（r =0.999 9）和0.2192～4.3840 μg（r=0.999 6）具有良好的线性关系,平均回收率（n=6）分别为96.57％ （RSD1.32％）和99.48％ （RSD 2.53％）.结论：该方法准确可靠,可用于狭基线纹香茶菜（溪黄草）药材质量控制.

Objective：To establish methods for identification and determination of caffeic acid and rosmarinic acid in Rabdosia.lophanthoides var gerardiana by TLC and HPLC.Method：The developing solvent for caffeic acid and rosmarinic acid in R.lophanthoides var gerardiana by TLC was n-hexane-ethylestate-formic acid （3∶3∶1）,detected under the UV light （365 nm）.HPLC method was performed on a Dikma Diamonsil C18 column （4.6 mm ×250 mm,5 μm） with a mobile phase of methanol-0.3% phosphoric acid by gradient elution.The flow rate was 1.0 mL ·min-1.The column temperature was kept at 25 ℃ and detection wavelength was set at 329 nm.Result：Caffeic acid and rosmarinic acid could be detected with clear spots and good separated by TLC,caffeic acid and rosmarinic acid showed good linearity in the ranges of 0.045 4-0.908 0 μg （r =0.999 9） and 0.219 2-4.3840 μg （r=0.999 6） with average recoveries of 96.57% （RSD 1.32%） and 99.48% （RSD 2.53%）.Conclusion：The methods are accurate and can be used for the quality of R.lophanthoides var gerardiana.