摘要
目的:建立RP-HPLC测定家蚕中1-脱氧野尻霉素(DNJ)的含量.方法:采用Hypersil NH2色谱柱(4.6 mm×250mm,5μm),以乙腈-水(78∶22)为流动相,流速1.0 mL·min-1,柱温30℃,检测波长203 nm.结果:家蚕中DNJ在1.996 ~9.98μg(r=0.999 6)峰面积与进样量呈良好的线性关系,平均加样回收率为98.4%.结论:该方法前处理简单、实用,结果准确,可用于家蚕及制品中DNJ的质量控制.
Objective:To establish assaying methods for the determination of deoxynojirimycin in silkworm.Method:The content of DNJ was determined by RP-HPLC,using a Hypersil NH2 (4.6 mm × 250mm,5 μm).The column temperature was maintained at 30 ℃.Acetonitrile-water (78:22) was used as mobile phase; the flow-rate was 1.0 mL ·min-1.The detecting wavelength was set at 203 nm.Result:The linear range was 1.996-9.98 μg (r =0.999 6).The average recovery was 98.4%.Conclusion:This method is simple,practical and accurate.It could be used for quality control of silkworm.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期124-126,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
福建省自然科学基金项目(2011D006)
厦门市科技计划项目(20124040)