高效液相色谱/串联质谱法测定肿节风分散片中异嗪皮啶含量

LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian

目的：建立高效液相色谱/串联质谱法测定肿节风分散片中异嗪皮啶.方法：采用Agilent SB-C18色谱柱（2.1 mm×50 mm,1.8μm）,流动相甲醇-水（35∶65）,流速0.2 mL· min-.三重四级杆质谱检测器,正离子多反应监测模式（MRM）,监测离子（m/z）222.9/189.7,222.9/161.9.结果：异嗪皮啶在0.48 ～2.40 mg·L-1与峰面积具有良好的线性关系（r =0.999 2）,平均加样回收率为99.3％,RSD 0.55％ （n=9）.结论：方法简便易行,结果准确,重复性好,可用于肿节风分散片中异嗪皮啶的含量测定.

Objective：To establish a method for determining isofraxidan in Zhongjiefeng Fensanpian by LC-MS-MS.Method：Agilent SB-C18 （2.1 mm ×50 mm,1.8 μm） column was used with methanol-H2O （35∶65） as mobilephase.The flow rate was 0.2 mL ·min-1.MS-MS detection in multiple reactions monitoring （MRM）mode using positive electrospray ionization as the ionization source was applied to determine positive ions at （m/z）222.9/189.7,222.9/161.9.Result：Isofraxidan was linear in the range of 0.48-2.40 mg ·L-1 （r =0.999 2）.The average recovery was 99.3%,and RSD was 0.55% （n =9）.Conclusion：The method is simple,rapid,accurate and reliable,which can be used for determination of isofraxidan in Zhongjiefeng Fensanpian.