HPLC测定小活络丸(浓缩丸)中11-羰基-β-乙酰乳香酸

Determination of Acetyl-11-keto-β-boswellic Acid in Xiaohuoluowan(Concentrated Pills) by HPLC

目的：建立小活络丸（浓缩丸）中11-羰基-β-乙酰乳香酸的高效液相色谱含量测定方法.方法：Inertsil ODS-3 C18（4.6 mm ×250 mm,5 μm）色谱柱,流动相乙腈-0.1％冰醋酸（80∶20）,流速1 mL· min-,检测波长249 nm,柱温30 ℃,外标法测定小活络丸（浓缩丸）中乳香主成分11-羰基-β-乙酰乳香酸.结果：11-羰基-β-乙酰乳香酸在25.95～207.6 mg·L-线性关系良好（R2=0.999 9）;平均加样回收率为99.95％,RSD 3.29％ （n=6）.结论：方法操作简便,结果准确,重复性良好,可用于小活络丸（浓缩丸）中乳香的质量控制.

Objective：To establish a high performance liquid chromatography （HPLC） method to determine acetyl-11-keto-β-boswellic acid in Xiaohuoluowan （concentrated pills）.Method：The determination was carried out with Inertsil ODS-3 C18 （4.6 mm × 250 mm,5 μm） column.The mobile phase consisted of acetonitrile-0.1% acetic acid （80∫ 20）.The flow rate was 1 mL ·min-1 The detection wavelength was set at 249 nm.The column temperature was set at 30 ℃.The external standard method was used to quantify acetyl-11-keto-β-boswellic acid,the main chemical constituent of olibanum in Xiaohuoluowan （concentrated pills）.Result：There was a good linear relationship between the absorption peak area and the concentration in the range of 25.95-207.6 mg ·L-1 （R2 =0.999 9） for acetyl-ll-keto-β-boswellic acid.The average recovery was 99.95% （n =6）.Conclusion：The method is simple,accurate and reproducible.It can be used for quality control of olibanum in Xiaohuoluowan （concentrated pills）.