高效液相色谱法测定白花丹参3种炮制品中5种酚酸类成分的含量

Simultaneous Determination the Five Phenolic Acids in the Three Processed Products of Salvia miltiorrhiza Bunge f. alba by HPLC

目的：建立同时测定白花丹参生品、酒制品和炒炭3种炮制品中5种酚酸类成分丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B含量的高效液相色谱法,并探讨不同炮制方法对白花丹参中主要酚酸类成分的影响.方法：大连依利特C18（4.6 mm×250 mm,5μm）柱;流动相甲醇-5％冰醋酸,梯度洗脱;流速1.0 mL·min-,检测波长286 nm,进样量20 μL.结果：丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别是7.31 ～234 （R2 =0.999 5）,6.75 ～216（R2=0.999 9）,52.8～1.65 （R2=0.9990）,4.67～ 149.50 （R2 =0.999 8）,42.25 ～1 352 mg·L-1 （R2 =0.999 9）;样品中5种酚酸类成分的平均加样回收率均＞98％,RSD均＜1.6％.结论：该方法简便快速,重复性好,灵敏度高,可作为白花丹参类药材的酚酸类成分含量的定量方法.测定结果显示,3种炮制品种均不含原儿茶酸,其余4种成分的含量均表现为生品＜酒制品＜炒炭.

Objective：To develop a HPLC method for simultaneous determination of danshensu,protocatechuic aicd,protocatechuic aldehyde,ferulic aicd and salvianolic aicd B in Salvia miltiorrhiza Bunge f.alba,the wine fried and the carbonizing,and explore the effects for their five phenolic acids with different processing methods S.miltiorrhiza Bunge f.alba.Method：The column was C18 （4.6 mm ×250 mm,5 μm）.The mobile phase was methanol and 5% acetic acid glacial with gradient elution and the flow rate was 1.0 mL ·min-1,the detection wavelength was 286 nm.Result：The linearities were obtained over the range of 7.31-234（R2 =0.999 5） for danshensu,6.75-216 （R2 =0.999 9） for protocatechuic acid,52.8-1.65 （R2 =0.999 0） for protocatechuic aldehyde,4.67-149.50 （R2 =0.999 8） for rosmarinic acid,42.25-1 352 mg ·L-1 （R2 = 0.999 9）for salvianolic aicd B,respectively.All of the average recoveries were 〉 98% with RSD 〈 1.6%.Conclusion：The validated method has the advantages of simplicity,precision and reliability,and could be used to simultaneous determination of the five phenolic acids in S.miltiorrhiza Bunge var alba.The results indicated that protocatechuic aicd wasn＇t detected in the three processed products,and the rest four contents presented the fresh 〈 the wine fried 〈 the carbonizing,and provided the theory foundation for pharmacy and clinic.