鳖甲中抑制单胺氧化酶活性肽筛选模型的建立

Establishment of the Screening Model for Active Peptide in Trionycis Carapax with Monoamine Oxidase-inhibitory Activity

目的：建立单胺氧化酶（MAO）活性筛选模型,以该模型指导鳖甲活性肽的分离,并检验分离单体活性.方法：参照MAO试剂盒方法,以优降宁为阳性药进行MAO活性筛选模型;对鳖甲水提液依次调节乙醇体积分数为20％,40％,60％,80％,90％,得到各醇沉部位,再以该模型筛选各醇沉部位,选取活性最优部分继续分离,然后将分得的单体以该模型检验活性.结果：该MAO活性筛选模型中,阳性药优降宁质量浓度在1.0g·L-1时,抑制率为98.30％ ～ 103.05％;各部位质量浓度为1.=g·L-1时,鳖甲水提液部位MAO抑制率为（23.34±9.66）％,20％醇沉部位（28.16±5.78）％,40％醇沉部位（30.69±7.17）％,60％醇沉部位（41.60±8.03）％,80％醇沉部位（64.91±2.94）％,90％醇沉部位（53.34±7.76）％;在80％醇沉部位分得鳖甲七肽（GAGPHGG）,质量浓度在0.1～1.6 g·L-1时,鳖甲七肽MAO抑制率为15.30％ ～54.84％.结论：发现MAO活性筛选模型优降宁抑制效果显著,提示该模型成功;以该模型成功指导了鳖甲七肽的分离,且鳖甲七肽具有较明显MAO抑制活性.

Objective：The monoamine oxidase （MAO） activity screening model is established to guide the separation of Trionycis Carapax active peptides,and inspect the activity of the monomer.Method：Pargyline was used as positive drug with reference to the method of MAO kit to establish the monoamine oxidase activity screening model; the ethanol concentration of Trionycis Carapax water extraction liquid was adjusting to 20%,40%,60%,80%,60% in turn to get the alcohol precipitated parts,then screen the alcohol sink parts in this model,and continue the separation of optimal selection activity part,finally MAO inhibitory activity of the monomer was tested in the model.Result：The pargyline＇s concentration was 1.0 g ·L-1,the inhibition rate on MAO was remarkable,which could amount to 98.30%-103.05% ; when the concentration was at 1.0 g ·L-1,the inhibitory rate of Trionycis Carapax water extraction liquid part on MAO was （23.34 ± 9.66） %,20% alcohol precipitated part was （28.16 ± 5.78） %,40% alcohol precipitated part was （30.69 ± 7.17） %,60% alcohol precipitated part was （41.60 ± 8.03）%,80% alcohol precipitated part was （64.91 ± 2.94）%,80% alcohol precipitated part was （53.34 ±7.76）%.We get the Trionycis Carapax peptide from the 90% alcohol precipitated part.The Trionycis Carapax peptide＇s inhibition rate on MAO was obvious when the concentration ranged from 0.1-1.6 g ·L-1,which ranged from 15.30% to 54.84%.Conclusion：The pargyline＇s inhibitory effect on MAO is remarkable,which suggests the model is successful and can guide separation of Trionycis Carapax,which has obvious inhibitory activity on MAO.