复方雪莲胶囊对Ⅱ型胶原诱导大鼠关节炎的治疗作用

Therapeutic Effect of Compound Xuelian Capsule on Type ⅡCollagen Induced Arthritis in Rats

目的:考察复方雪莲胶囊对Ⅱ型胶原诱导的类风湿性关节炎大鼠的治疗作用,并探讨其作用机制。方法:采用大鼠足趾部皮内注射牛Ⅱ型胶原乳剂,建立胶原诱导的关节炎模型(collagen-induced arthritis,CIA),设正常对照组、模型组、风湿定胶囊组(0.1 g·kg-1)、复方雪莲胶囊(0.05,0.1,0.2 g·kg-1)3个剂量组,于造模第14天分组并开始灌胃给药,连续给药14 d。观察复方雪莲胶囊对CIA大鼠关节肿胀的治疗作用;取关节滑膜组织做病理学检查;并用酶联免疫(ELISA)法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)的水平。结果:造模14 d后,模型大鼠的足趾肿胀明显,与正常对照组比较,有显著性差异(P<0.01)。复方雪莲胶囊组可明显减轻CIA大鼠的足肿胀度,与模型组比较有显著性差异(P<0.05,P<0.01);各给药组关节滑膜组织病变与模型组比较明显改善;与模型组大鼠血清中TNF-α(51.3±16.8)ng·L-1及PGE2(683.3±174.7)ng·L-1的水平比较,复方雪莲胶囊(0.1,0.2 g·kg-1)剂量组大鼠血清中TNF-α[(24.5±13.9),(23.1±7.0)ng·L-1]及PGE2[(452.9±102.2),(466.8±88.4)ng·L-1]的水平明显降低(P<0.01)。结论:复方雪莲胶囊对大鼠胶原性关节炎有一定的抑制作用,其作用机制可能与抑制炎症介质TNF-α及PGE2的表达有关。

Objective：To investigate the therapeutic effect of compound Xuelian capsule on type Ⅱ collagen-induced arthritis in rats,and to explore its mechanism.Method：Rats were given pedal intradermal injection of bovine type Ⅱ collagen emulsion,collagen-induced arthritis （CIA） model was established.To measure and observe the effect of compound Xuelian capsule on the ankle swelling of CIA rats,the synovial tissue was collected to observe the synovial histopathology of rats.The phlogistic cell factor tumor necrosis factor-α （TNF-α） and prostaglandin E2 （PGE2） were detected by enzyme linked immunosorbent assay （ELISA）.Result：In model group,the rat paw edema was significant compared with the normal control group （P 〈 0.01）.Compound Xuelian capsule could significantly decrease the arthritis swelling of CIA rats （P 〈0.05,P 〈0.01） ; in compound Xuelian capsule groups,it could improve the synovial tissue pathological damage,compared with the CIA model group,it could significantly reduce inflammatory cell infiltration and hyperemia of synovial tissue.After 14 days＇administration,the level of TNF-α and PGE2 was significantly lower in the medium and high dose group of compound Xuelian capsule than that in the model group （P 〈0.05,P 〈0.01）.Conclusion：Compound Xuelian capsule could inhibit the collagen-induced arthritis in rats,the mechanism action may be related to inhibiting the expression of inflammatory mediators TNF-α and PGE2.