滋阴清热方对金黄地鼠血清性激素及雄激素受体mRNA表达的影响

Effect of Ziyin Qingre Decoction on Serum Levels of Sex Hormones and Androgen Receptor mRNA Expression in Golden Hamster

目的:探讨滋阴清热方对金黄地鼠血清性激素及雄激素受体表达的影响。方法:选用成年雄性金黄地鼠侧腹皮脂腺斑作为动物模型,随机分为4组,分别予生理盐水、滋阴清热方高剂量(18 g·kg-1)、滋阴清热方低剂量(9 g·kg-1)、安体舒通(10 mg·kg-1)灌胃给药,每天1次。4周后,采用ELISA法检测各组血清睾酮(T)、雌二醇(T2)的水平,实时荧光定量PCR法检测各组皮脂腺斑处雄激素受体(AR)mRNA的表达水平。结果:滋阴清热方高、低剂量组与空白组比较,能降低血清T水平,但无显著性差异;高剂量组血清E2水平升高,与空白组比较有显著性差异(P<0.05)。4组皮脂腺斑处AR mRNA的表达水平有显著性差异(P<0.05),清热滋阴方高剂量组与空白组比较有显著性差异(P<0.05)。结论:滋阴清热方可能是通过改善金黄地鼠血清雌二醇水平,并抑制局部雄激素受体mRNA的表达,而影响皮脂腺的增生。

Objective：To investigate the effect of Ziyin Qingre decoction on golden hamster in serum sex hormones and adnrogen receptor.Method：The animals were randomly divided into 4 groups,namely blank control group,positive control group,high and low dosage Ziyin Qingre decoction.Rats were respectively fed with saline solution,antisterone （10 mg ·kg-1）,Ziyin Qingre decoction （18,9 g ·kg-1） for 4 weeks.The serum testosterone （T） and estradiol （E2） levels were detected using enzyme-linked immunosorbant assay （ELISA）method.The gene androgen receptor （AR） mRNA in flank organ was measured by real-time PCR.Result：Compared with blank control group,high and low dosage Ziyin Qingre decoction could reduce serum T level and serum E2 level （P 〈 0.05）.There was a difference between four groups in expressions of AR mRNA （P 〈0.05）.The AR mRNA in high dosage group was decreased （P 〈 0.05）,compared to the blank control group.Conclusion：Ziyin Qingre decoction can increase the serum E2 level and suppress the expression of AR mRNA.These may affect the proliferation of sebaceous gland.