补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究

Experimental Research on PI3K/AKT Expression Mechanisms of Buzhong Yiqi Decoction in Nude Mice Lung Adenocarcinoma Transplantation Tumor

目的:探讨补中益气汤对裸鼠肺腺癌移植瘤生长抑制作用及对磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI3K)/蛋白激酶B(protein kinase B,AKT)信号通路的影响。方法:50只裸鼠随机分为①正常对照组,②荷瘤对照组,③顺铂组,④顺铂联合补中益气汤组,⑤补中益气汤组。分别给予相应干预后,处死小鼠,计算抑瘤率;应用免疫组化方法、实时定量PCR(real time PCR,RT-PCR)检测移植瘤组织中PI3K,AKT的表达水平。结果:顺铂联合补中益气汤组对移植瘤的抑制率最强,与其他各组比较有显著差异(P<0.05),顺铂和补中益气汤协同作用的q值1.201;移植瘤中PI3K,AKT蛋白和mRNA基因的表达在顺铂联合补中益气汤组显著降低(P<0.05)。结论:补中益气汤联合顺铂对耐顺铂肿瘤有协同抑制作用;补中益气汤能通过干预PI3K、AKT基因和蛋白的表达而增加顺铂对耐顺铂肿瘤的敏感性。

Objective：To discuss the effect on lung adenocarcinoma transplantation tumor in nude mice and the influence of phosphatidylinositol 3 kinase/protein kinase B （PI3K/AKT） signaling pathway of Buzhong Yiqi decoction.Method：50 nude mice were randomly divided into 5 groups,intervented respectively after 14 days,put to death and calculating tumor inhibition rate and used immunohistochemical method and real time PCR （RT-PCR） detecting the expression level of PI3K,AKT in transplantation tumor tissue.Result：Conbined group was the strongest inhibition rate of transplanted tumor,compared with other groups,there are statistically significant （P 〈 =.05）.The q value of synergistic effect of cisplatin and Buzhong Yiqi decoction was 1.201.PI3K and AKT protein and gene mRNA expression in conbined group were decreased.There are statistically difference among other groups （P 〈 0.05）.Conclution：The Buzhong Yiqi decoction combined with cisplatin has synergistic inhibitory effect in Cisplatin resistance tumor.The Buzhong Yiqi decoction can increase cisplatin resistance mechanism throught regulating the gene and protein expression of PI3K and AKT.