黄芪配伍不同剂量柴胡对脾虚发热证模型的作用

Effect of Compatibility of Astragalus and Different Dose Radix Bupleuri on the Model of Spleen-qi Deficiency with Fever in Rats

目的:探讨黄芪配伍不同剂量柴胡对脾虚发热大鼠模型的作用。方法:雄性Wistar大鼠随机分为正常对照组、脾虚发热模型组、芪柴高、中、低剂量组、高剂量柴胡组共6组。采用饮食失节+游泳疲劳制备脾虚模型。实验第17天开始,各给药组大鼠分别按剂量4.56,2.95,2.41,2.41 g·kg-1ig,正常组和模型组同体积蒸馏水ig,1次/d,连续6 d。第22天模型组和各给药组以脂多糖(LPS,80μg·kg-1)ip,正常对照组等量生理盐水ip。观测各组大鼠每周的外观行为变化和LPS ip后不同时间点的体温,测定外周血相关脑肠肽的含量。结果:与正常组相比,模型组大鼠在LPS ip后各点体温均见不同程度升高,60,220 min时升高明显(P<0.05);外观行为积分显著升高(P<0.05),外周血生长抑素(SS)、胆囊收缩素(CCK-8)、β内啡肽(β-EP)均显著升高(P<0.05)。与脾虚发热组相比,各给药组大鼠体温均见不同程度下降(P<0.05),其中柴胡组和芪柴中、低剂量组体温下降明显,体温曲线明显偏离正常组;各给药组中仅芪柴中、低剂量组的部分行为指标及积分下降明显(P<0.05);各给药组血β-EP均显著升高(P<0.05),芪柴各剂量组血SS和CCK-8均呈不同程度的降低作用趋势,但高剂量柴胡组SS和CCK-8反见升高趋势。与高剂量柴胡组比较,芪柴各剂量组血SS均显著降低(P<0.05),芪柴高剂量组血β-EP显著降低(P<0.05),芪柴中、低剂量组血CCK-8显著降低(P<0.05)。结论:黄芪配伍不同剂量柴胡对脾虚发热模型均有退热作用,对外观行为或相关脑肠肽均有不同程度的改善作用;单用高剂量柴胡仅有退热作用;综合效应以黄芪配伍低剂量柴胡的防治效果最好。

Objective：To investigate the effect of compatibility of Astragalus and different dosage of Radix Bupleuri on the model of spleen-qi deficiency with fever in rats.Method：The male Wistar rats were assigned into the normal control group modle group,the spleen-qi deficiency with fever group （model group）,the Astragalus with high dosage Radix Bupleuri group,the Astragalus with medium dosage Radix Bupleuri group,the Astragalus with low dosage Radix Bupleuri group,and the only Radix Bupleuri group.The rats in model group and medicine groups were treated with excess fatigue plus out of constant diet for 21 days.From the 17th day,the medicine group rats were fed with 4.56,2.95,2.41 g ·kg-1 and 2.41 g ·kg-1 （Radix Bupleuri only） once a day and the other two groups were fed with physiological saline.On the 22th day,the rats in model group and medicine groups were respectively intraperitoneally injected with lipopolysaccharide （LPS,80 μg ·kg-1） to induce fever.The rats of the normal group were injected with physiological saline.The appearance and behavior characteristics of rats were observed twice a week and the rectal temperature was measured at different time after ip LPS.The content of braingut petide in peripheral blood was also measured.Result：Compared with the normal group,the rectal temperature of the spleen-qi deficiency with fever group raised at each time point in different degree after ip LPS,especially at 60,220 min （P 〈 0.05）; the integration of appearance and behavior characteristics increased significantly （P 〈 0.05） ; somatostatin （SS）,cholecystokinin-8 （CCK-8）,β-endomorphin （β-EP） in peripheral blood increased significantly （P 〈 0.05）.Compared with model group,the rectal temperature of every medicine group were decreased significantly in different degree （P 〈 0.05）,especially in the only Radix Bupleuri group and the Astragalus with low dosage Radix Bupleuri group,the temperature curve deviated from the normal group ; the integration of appearance and behavior characteristics decreased significantly （P 〈 =.05） only in the Astragalus with medium and low dosage Radix Bupleuri groups ; serumβ-EP increased significantly （P 〈 0.05） in all medicine groups; serum CCK-8,SS showed a downward trend in the Astragalus with high,medium and low dosage Radix Bupleuri groups,but sexum CCK-8,SS in the only Radix Bupleuri group showed a rising trend.Compared with the only Radix Bupleuri group,serum SS decreased significantly （P 〈 0.05） in the Astragalus with high,medium and low dosage Radix Bupleuri groups; serum β-EP decreased significantly （P 〈 0.05） in the Astragalus with large dosage Radix Bupleuri group; serum CCK-8 decreased significantly （P 〈 0.05） in the Astragalus with medium and low dosage Radix Bupleuri groups.Conclusion：The compatibility of Astragalus and different dosage of Radix Bupleuri has antipyretic effect on the model group and the integration of appearance and behavior characteristics and braingutpetide were improved in different degrees.The only Radix Bupleuri group has the antipyretic effect only.The Astragalus with low dosage Radix Bupleuri group has the best controlling effect for the comprehensive indices.