半枝莲多糖对S180荷瘤小鼠红细胞功能的影响

Effect of Scutellaria barbata Polysaccharide on the Function of Erythrocyte Membrane in S180 Tumor-bearing Mice

目的:探讨半枝莲多糖增强荷瘤小鼠红细胞膜流动性及其红细胞免疫调节机制。方法:健康雄性小鼠48只,按体重随机分为正常对照组、模型对照组、黄芪多糖对照组(100 mg·kg-1)、半枝莲多糖低、中、高剂量组(50,100,200 mg·kg-1)。用荧光分光光度计检测荷瘤小鼠红细胞膜流动性;用紫外分光光度计检测红细胞膜Ca2+,Mg2+-ATP酶活力、唾液酸(SA)含量、抗氧化酶系的活力。结果:与模型对照组比较,半枝莲多糖各剂量组可明显提高S180荷瘤小鼠红细胞膜流动性(P<0.01,P<0.05),可明显提高红细胞膜Ca2+,Mg2+-ATP酶活力(P<0.01,P<0.05)、及抗氧化酶系的活力(P<0.01,P<0.05);低、中剂量组可增加红细胞膜表面唾液酸含量(P<0.01)。结论:半枝莲多糖可能通过改善S180荷瘤小鼠红细胞膜的功能状态,提高膜的流动性,增强红细胞的免疫功能,从而发挥其抗肿瘤作用。

Objective：To study the effect of Scutellaria barbata polysaccharide on the fluidity of erythrocyte membrane and its mechanism of red cell immune regulation.Method：Fourty eight mice were randomly divided into six groups：normal control group,model control group,Astragalus polysaccharide group （100 mg ·kg-1）,S.barbata polysaccharide Dou low,middle,and high dose group （50,100,200 mg ·kg-1）.The membrane fluidity of erythrocytes in tumor-bearing mice was determined by fluorescence spectrophotometry; the erythrocyte membrane Ca2 ＋,Mg2 ＋-ATPase activity sialic acid （SA） content,antioxidant enzymes activity were detected by UV spectrophotometer.Result：Compared with the modle group,S.barbata polysaccharide low dose,middle dose,high group could obviously improve the fluidity of erythrocyte membrane in S180 tumor-bearing mice （P 〈0.01,P 〈0.05）,obviously increase the red cell membrane Ca2＋,Mg2＋-ATPase activity （P 〈 0.01,P 〈0.05） and antioxidant enzymes activity （P 〈 0.01,P 〈 0.05） ; low,middle group increased sialic acid content of red cell membrane surface （P 〈 0.01）.Conclusion：S.barbata polysaccharide can increase the fluidity of erythrocyte membrane and enhance the immune function of erythrocyte in S180 tumor-bearing mice,thus it may exert aiti-tumor effect by improving the function of erythrocyte membrane.