慢性丙型肝炎患者丙肝病毒感染与载脂蛋白B关系的初步研究

Correlation of Apolipoprotein B and Hepatitis C Virus Infection in Patients with Chronic Hepatitis C

目的:初步研究慢性丙型肝炎(CHC)患者丙肝病毒(HCV)感染与载脂蛋白B(ApoB)之间的关系。方法:以78例CHC患者(CHC组)和60例健康体检者(对照组)为研究对象,用生化分析仪测定其血清低密度脂蛋白(LDL)、载脂蛋白B(ApoB)水平;用荧光定量PCR法测定血清HCV-RNA病毒载量,并据此将CHC患者分为低载量组(<107copies/ml)和高载量组(≥107copies/ml);用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测ApoB启动子区-516C/T基因多态性,统计分析各指标变化及相互关系。结果:与对照组比较,CHC组血清LDL、ApoB水平显著降低(P<0.05),ApoB启动子区-516位点的CC基因型频率明显升高(P<0.05);与携带TT基因型的CHC患者比较,携带CC基因型的CHC患者血清ApoB水平明显降低(P<0.05);与低载量组患者比较,高载量组患者血清ApoB水平显著降低(P<0.05),两者呈负相关(r=-0.298,P<0.01)。结论:低ApoB水平可增加HCV感染;其启动子区-516C/T位点的C等位基因可能与HCV的易感性相关。

Objective:To investigate the correlation of apolipoprotein B(ApoB) and HCV infection in patients with chronic hepatitis C.Method:78patients with chronic hepatitis C and 60healthy volunteers were selected. The levels of serum LDL and ApoB were measured using a biochemical autoanalyzer.Serum HCV-RNA virus load was detected by fluorescence quantitative PCR method,according to HCV-RNA virus load,the patients with CHC were divided into low(<107copies/ml),and high(≥ 107 copies/ml)virus load groups.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique was used to analyze the ApoB genotypes in the promoter region-516.Results:The levels of serum LDL and ApoB in patients with chronic hepatitis C were significantly lower than those of normal subjects(P<0.05).The frequency of CC genotype in the ApoB promoter region-516in patients with chronic hepatitis C was significantly higher than that of healthy control group(P <0.05).Among the patients with chronic hepatitis C,the serum level of ApoB in TT genotype carriers was higher than that in CC genotype carriers(P<0.05).The serum level of ApoB in different virus load groups were significantly different,the level of ApoB in low virus load groups was significantly higher than in high virus load groups(P<0.05);the level of serum ApoB was negatively correlated with HCV-RNA virus load(r=-0.298,P<0.01).Conclusion:The level of serum ApoB in patients with chronic hepatitis C was significantly decreased.Polymorphism of ApoB gene in promoter region-516was associated with HCV infection,C allele may be genetic factor for HCV infection.