淫羊藿对糖皮质激素大鼠肾脏损害的预防作用及对骨形态发生蛋白-7的影响

Epimedium prevented glucocorticoid- induced kidney damages by regulation of bone morphogenetic protein- 7 in rats

目的观察醋酸泼尼松对大鼠肾脏组织形态学和功能的影响,探讨淫羊藿(EP)对糖皮质激素(GC)大鼠肾脏损害的预防作用及对骨形态发生蛋白-7(BMP-7)的影响。方法 3月龄SD大鼠24只随机分为3组:正常对照(Cont组)组、糖皮质激素组(GC组)和淫羊藿防治组(GC+EP组),均为上午选模给药,下午防治给药,灌胃给药90 d。苏木素-伊红染色法观察肾脏组织形态学,分光光度计比色法测定血清肌酐和尿素氮(BUN)含量,免疫组化法检测肾脏BMP-7的表达。结果 GC组大鼠部分肾小球明显萎缩,个别肾小管萎缩,少数肾小管有蛋白管型,BUN含量增高(P<0.01),肾脏BMP-7表达减少(P<0.01);与GC组比较,GC+EP组肾小球基本正常,肾小管未见明显萎缩,无蛋白管型的发生,BUN含量和肾脏BMP-7表达正常(P<0.001)。结论EP可通过上调肾脏BMP-7表达预防GC所致的肾脏结构和功能损害。

Objective To observe the renal histomorphologicaland functional change induced by glucocorticoid （GC） in rats, and to further investigate the protective effects of epimedium （EP） on GC - induced kidney damages by regulating bone morphogenetic protein - 7 （ BMP - 7 ）. Methods Twenty - four SD rats of 3 - month were randomly divided into three groups： the normal group （Group C ） , the GC group （Group G） and the GC plus EP treatment group （ Group T） ;in which all drugs were given for 90 days. Hematoxylin - eosin （HE） staining was used for renal pathological observation, while spectrophotometer was applied for assessment of serum creatinine and blood urea nitrogen, and immuno- histochemical analysis was used to assessthe expression of kidney BMP - 7. Results Notable aeinusrenisatrophia, morderate renal tubule atrophia, and several protein cast inrenal tubules were observed with significant elevation of BUN and down - regulation of BMP -7 in Group G （P 〈0. 01 ） ; which were significantly reversed by EP treatment in Group T（P 〈 0. 001 ）. Conclusion EP can prevent GC - induced kidney damages in rats by up - regulating renal BMP - 7 expressions.