摘要
叶片是光合作用的重要器官,适度卷曲有利于改善群体光照、提高光能利用率,卷叶基因是培育理想株型的重要资源。为研究控制水稻叶片形态建成的分子机制,从EMS诱变粳稻品种日本晴的M2代中分离了一个叶片向内卷曲的突变体s1-145,该突变体叶绿素含量增高,株高和育性等产量性状正常。遗传分析表明该性状受1对隐性基因控制。利用InDel标记将该基因定位于第2染色体R2-34.70和R2-34.79之间物理距离为90 kb的范围内。本研究结果为该卷叶基因的克隆和功能分析奠定了基础,对水稻株型改良提供了基因资源和育种材料。
Leaf is an important organ for photosynthesis. Moderate rolling of leaves can facilitate the improvement of plant's population structure and enhance light-use efficiency, which is very important in ideotype breeding of rice. In the present study, in order to systematically dissect the molecular mechanism of leaf morphogenesis and development, one ethyl methylsulfone (EMS) -induced rice (Oryza sativa L.) mutant with adaxially-rolled leaf, namely s1-145, was characterized. This mutant exhibited higher chlorophyll content, normal plant height and fertility. Genetic analysis indicated that the mutant was controlled by a single recessive gene. The mutated gene of s1-145 was fine mapped within a 90 kb interval between two InDel markers R2-34.70 and R2-34.79 on the long arm of chromosome 2 in rice. These results provide a basis for the final cloning and functional analysis of the leaf-rolling gene, as well as gene resource and plant material for rice ideotype breeding.
出处
《作物学报》
CAS
CSCD
北大核心
2013年第11期1970-1975,共6页
Acta Agronomica Sinica
基金
国家转基因生物新品种培育科技重大专项(2011ZX08009-003)资助