水稻细胞悬浮系的中短期保存方法

Short-medium Term Preservation of Rice Suspension Cells

细胞悬浮系的中短期保存广泛需要。本文应用中花15细胞悬浮系，比较了AA、N6、MS三种固体培养基对水稻细胞悬浮系的繁殖保存效果，结果表明，AA固体培养基保存效果最好。细胞系可以在AA。培养基上4个月、在AA0.5培养基上6个月（中间45d左右继代一次）连续保存后仍然保持其可重悬性。通过比较AA固体培养基繁殖保存、冷冻保存、连续悬浮培养3种保存方法，表明AA固体培养基繁殖保存细胞系2～9个月内，既可保持细胞的可悬浮性，又对细胞系的POD、SOD活性和植株再生率影响较小，是一种理想的细胞悬浮系中短期保存方法。

The preservation of cell suspension in short-medium term is widely needed in cell biology. In this paper, the preserva- tion effects of cell suspension of rice cultivar Zhonghua 15 on AA, N6, and MS solid culture media were compared. The results showed that AA medium was suitable for suspension cells preservation. The cell suspension still kept resuspensible after growing continuously on AA solid medium for 4 months, and then on AA medium supplemented with 0.5 mg L-1 2,4-D for 6 months （subcultured at about 45 d interval）. Three kinds of methods of suspension cells preservation, namely preservation on AA solid medium, cryopreservation, and continuously suspending culture, were compared. The results indicated that the first method kept good suspensible property, had high regeneration rate, and had a little influence on activities of POD and SOD, even though the suspension cells grew on AA medium for 2-9 months. AA solid medium is ideal for the preservation of suspension cells in short-middle-term.