非小细胞肺癌组织中PTEN基因和Ki67蛋白的表达及意义

Expression and significance of PTEN gene and Ki67 protein in non-small cell lung cancer

目的探讨第10染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)和Ki67蛋白在肺癌组织中的表达及其临床意义。方法采用免疫组化方法检测67例非小细胞肺癌(NSCLC)组织(A组)及41例癌旁正常肺组织(B组)中PTEN基因和Ki67蛋白的表达。结果 A组PTEN基因阳性表达率显著低于B组(32.84%vs.82.93%),而Ki67表达则显著高于B组(76.12%vs.27.31%)(P<0.05)。PTEN基因和Ki67的表达与肺癌的TNM分期、临床分期、淋巴结转移和4年生存率密切相关(P<0.05)。NSCLC的PTEN基因表达与Ki67呈负相关(r=-0.239,P<0.05)。Kaplan-Meier生存曲线分析显示,NSCLC的PTEN表达下调和Ki67表达上调者,术后生存率下降。结论联合检测PTEN与Ki67的表达有助于判断非小细胞肺癌的预后。

Objective To study the expressions and significance of tenth chromosome phosphatase and tension homology deleted protease gene（PTEN gene） and Ki67 protein in non-small cell lung cancer （NSCLC）. Methods The expressions of PTEN and Ki67 were detected by immunohistochemistry in NSCLC of 67 patients（group A） and tumor-adjacent normal lung tissues of 41 samples（group B）. Results The positive expression rate of PTEN in group A was significantly lower than that in group B （32.84% vs. 82.93%）（P〈0. 05）. The expression of Ki67 was significantly higher in group A than that in group B（76.12% vs. 27.31%） （P〈0.05）. The expressions of PTEN and Ki67 were closely correlated with TNM grade, clinical stage, lymph node metastasis and 4-year survival rate after surgery（P〈0. 05）. The expression of PTEN was negatively correlated to that of Ki67 （r=- 0. 239, P〈0. 05）. Kaplan-Meier analysis showed that downregulated expression of PTEN and upregulated expression of Ki67 were associated with lower survival rate in NSCLC patients after surgery. Conclusion Combined detection of the expressions of PTEN and Ki67 in NSCLC can be taken as the indicators for evaluating the prognosis of NSCLC patients.