摘要
目的探讨慢性活动性Epstein-Barr病毒(Epstein-Barr virus,EBV)感染、急性EBV感染及正常儿童EBV-DNA及适应性体液免疫的差异。方法慢性活动性EBV感染患儿8例(慢性组),急性EBV感染患儿13例(急性组),正常儿童12例(对照组)外周血单个核细胞采用实时荧光定量PCR法检测,3组EBV-DNA水平,采用ELISA、抗体稀释试验评价EBV适应性体液免疫,分析其与EBV感染不同转归的关系。结果慢性组EBV-DNA载量、病毒壳蛋白抗原-IgA、-IgG及早期抗原-IgA水平明显高于急性组和对照组(P<0.01),病毒壳蛋白抗原-IgM水平及早期抗原-IgG滴度改变速率低于急性组(P<0.01);慢性组与急性组EBV核抗原-IgG抗体水平均低于对照组(P<0.01)。结论 慢性活动性EBV感染存在不同EBV核抗原-LP拷贝数及不同亲和力的抗体谱,可能与慢性活动性病程相关,对早期识别诊治有重要意义。
Objective To study the Epstein-Barr virus （EBV）-DNA quantitative level and the differences in adaptive immune reactions in pediatric patients with chronic active EBV infection （CAEBV）, acute EBV infection （AEBV） and normal children. Methods Real-time quantitative PCR was used to detect the EBV DNA levels in peripheral blood mononuclear cells （PBMC） in 12 normal children, 8 pediatric patients with CAEBV and 13 pediatric patients with AEBV. ELISA and antibody affinity assays were used to evaluate EBV specific adaptive humoral immunity. The relationship between the above results and the prognosis of EBV infection was analyzed. Results The EBV-DNA load, viral capsid antigen-IgA, -IgG, early antigen-IgA levels in PBMC were significantly higher in CAEBV patients than those in AEBV patients and normal children （P〈0.01）. The levels of viral eapsid antigen-IgM and the change of early antigen-IgG titres were lower in CAEBV patients than those in AEBV patients （P〈0.01）. EBV nuclear antigen-IgG level was significantly lower in CAEBV and AEBV patients than that in normal children （P〈0.01）. Conclusion The difference of the EBNA- LP-DNA quantitative level and the different type of EBV-antibodies of different affinity may be associated with the chronic active re-infection process, which may play an important role in the early recognition, diagnosis and treatment of CAEBV.
出处
《中华实用诊断与治疗杂志》
2013年第11期1070-1072,共3页
Journal of Chinese Practical Diagnosis and Therapy
