2009H1N1流感病毒对A549细胞和BEAS-2B细胞作用的初步研究

Preliminary study on cellular effect of A549 and BEAS-2B cells infected by 2009H1N1 influenza virus

目的探求2009H1N1流感病毒对A549细胞和BEAS-2B细胞作用,为研究2009H1N1流感病毒的致病机理提供线索。方法不同来源(死亡、重症、普通病例分离)的2009H1N1流感病毒和季节性H1N1流感病毒分别感染A549和BEAS-2B细胞12、24、48、72h后用流式细胞术检测细胞凋亡和细胞周期。结果感染A549细胞12和24h,普通病例分离的2009H1N1流感病毒组的细胞凋亡率最高(P<0.05),重症组的细胞凋亡率最低(P<0.05);48h和72h,死亡组细胞凋亡率最高(P<0.05)。感染BEAS-2B细胞12h,重症组细胞凋亡率最高(P<0.05);48h,死亡组和重症组细胞凋亡率高(P<0.05);72h,死亡组和普通组细胞凋亡率高(P<0.05)。4株病毒主要将A549细胞阻滞在S期,将BEAS-2B细胞阻滞在G0/G1期。结论在细胞凋亡和细胞周期的细胞学观察水平上2009H1N1流感病毒和季节性H1N1流感病毒之间存在差异,不同来源的2009H1N1流感病毒之间也存在差异。

In the study, we aim to detect the effect of A549 and BEAS-2B cells infected by 2009H1N1 influenza virus and to provide clues for the study of pathogenicity mechanism of 2009H1N1 influenza virus. A549 and BEAS 2B cells were in- fected by 2009H1N1 influenza viruses （isolated from death cases, severe cases, and ordinary cases） and the seasonal H1NI in fluenza virus, respectively. Cells were monitored by flow cytomery to detect cell apoptosis and cell cycle after 12, 24, 48 and 72 hours post-infection with viruses. The highset apoptotlc percentage of A549 cells in 12 and 24 hours post infection was in viru ses isolated from ordinary case （P〈0.05）, simultaneously. The viruses isolated from severe case was the lowest （P%0.05）, and that isolated from death cases was the hightest after 48 and 72 hours post infection （P%0.05）. The highest apoptotic per- centage of BEAS-2B cells in 12 hours post-infection was viruses isolated from ordinary case （P〈0.05） ; that of viruses isolated from death and severe cases were higher after 48 hours post-infection （P%0.05）; the viruses isolated from death and ordinary cases were high in 72 hours post infection （P〈0.05）. Results suggest that the A549 cells are inhibited in Sphase post infec- tion, however, BEAS-2B cells are inhibited in G0/G1 phase. There are differences between 2009H1N1 influenza virus and sea- sonal H1N1 influenza virus in the cytological level of cell apoptosis and ceil cycle, simultaneously, and there are differences a- mong 2009H1N1 influenza viruses.