2型糖尿病患者红细胞膜流动性和血浆自体荧光变化的实验研究

Research on red cell membrane fluidity and plasma autofluorescence of type 2 diabetes mellitus

目的探讨脂代谢紊乱在2型糖尿病中的表现和作用。方法将2型糖尿病患者分为伴有高胰岛素血症(hyperinsulinemia,HINSE)和不伴有HINSE两组;用荧光偏振技术测量红细胞膜的流动性;用荧光分光光度计测量血浆的自体荧光发射光谱。结果伴HINSE组较不伴HINSE组,红细胞膜的微粘度增大,流动性降低;糖尿病患者在445 nm的荧光峰明显增强,且伴HINSE组较不伴HINSE组增强明显。结论 2型糖尿病患者高胰岛素血症导致脂质代谢紊乱,红细胞变形性下降,膜流动性和通透性改变。脂质过氧化反应和血脂异常导致血浆自体荧光光谱改变。

Objective To explore the performance and role of lipid disorders in type 2 diabetes mellitus （DM）. Methods Patients with type 2 DM were divided into 2 groups： with hyperin- sulinemia （HINSE） and without HINSE. The membrane fluidity of red blood cell was meas- ured by fluorescence polarization technology. The fluorescence emission spectrums were meas- ured and analyzed by spectrophotofluorometer. Results Compared with type 2 DM without HINSE, the micro viscosity of red cell membrane was increased, liquidity was reduced; the fluorescence peak in 445 nm increased obviously, which of type 2 DM with HINSE increased more significantly than those of without HINSE. Conclusion Type 2 DM with HINSE leads to lipid disorders, decrease of red cell deformability, membrane fluidity and permeability. Lipid peroxidatic reaction and blood fat abnormality lead to plasma auto-fluorescence changing.