乳化氟碳保存液对大鼠供肺的保护作用

Protective effect of perfluorocarbon emulsions on rat donor lung

目的 评价乳化氟碳保存液对大鼠供肺的保护作用.方法 健康雄性SD大鼠24只,体重350 ～ 400 g,采用随机数字表法,将其分为2组（n=12）：UW液组（UW组）和乳化氟碳保存液组（FCE组）.建立肺灌注模型后取下心肺,置于4℃UW液或乳化氟碳保存液中.于6h时取肺组织,采用WST法测定SOD活性,TBA法测定MDA含量,ELISA法测定MPO活性和IL-1β、IL-6、TNF-α的含量,并观察肺组织病理学结果.结果 与UW组比较,FCE组肺组织MPO活性降低（P＜0.05）；2组肺组织SOD活性及MDA、IL-1β、IL-6和TNF-α的含量比较差异均无统计学意义（P＞0.05）.结论 乳化氟碳保存液可降低肺组织中性粒细胞的浸润程度,提示其在减轻供肺损伤方面较UW液更具优势.

Objective To evaluate the protective effect of perfluorocarbon emulsions （FCE） on donor lung of rats during storage.Methods Twenty-four healthy male Sprague-Dawley rats,weighing 350-400 g,were equally and randomly divided into 2 groups using a random number table：University of Wisconsin （UW） solution group （UW group） and FCE group （FCE group）.After the model of lung perfusion was established according to the method described by Fischer et al,the lung and heart were removed and perfused with 4 ℃ UW or FCE preservation solutions.The lung was taken out when stored for 6 h for determination of SOD activity （by WST assay）,malondialdehyde （MDA） content （by TBA assay）,and activity of myeloperoxidase （MPO） and content of interleukin1 β （IL-1β）,IL-6,tumor necrosis factor-apha （TNF-α） （using ELISA） in lung tissues and for microscopic examination of pathologic changes.Results MPO activity was significantly lower in UW group than in FCE group （P 〈0.05）.There were no significant differences in the SOD activity and content of MDA,IL-1β,IL-6,TNF-α between the two groups （P 〉 0.05）.Conclusion FCE can reduce the neutrophil infiltration in lung tissues,indicating that FCE is more superior to UW solution in reduction of injury to the donor lung of rats. Keywords：Fluorocarbons ; Emulsifying agents; Lung transplantation; Cytoprotection