贵州地道药材淫羊藿rDNA ITS序列的分析研究

Analysis on Epimedium rDNA ITS Sequence of Authentic Medicinal Materials in Guizhou

[目的]通过对贵州桐梓淫羊藿rDNA ITS序列(包括ITS1、5.8S和ITS2)的测序分析,获得地道药材淫羊藿在分子水平上鉴定的参考标准。[方法]用改良的CTAB法提取淫羊藿总DNA,PCR扩增ITS序列后直接测序,并与Genbank中其他地区淫羊藿种的ITS序列进行序列同源性分析。[结果]淫羊藿rDNA ITS序列长约703 bp,其中ITS1长249 bp,ITS2长247 bp,序列间共有16个变异位点。[结论]淫羊藿rDNA ITS序列的确定可应用于地道药材淫羊藿的鉴定,可作为从分子水平进行鉴定中药淫羊藿的分子标记之一。

[ Objective ] The aim was to obtain the qualification standard of Epimedium which was an authentic herb grown in Tongzi Couty, Guizhou province based on the m01ecule level,by analyzing the rDNA ITS （including ITS1,5.8Sand ITS2） sequences o^Epimedium. [Meth- od ] The integrated DNA was extracted using modified CTAB. In addition, a homology analysis of ITS sequence and ITS Which was a main spe- cies of Epimedium in Genebank, would be presented after the direct sequence of PCR expanded to ITS. [ Result] The length of Epimedium rD- NA ITS Seqt^enCe was about 703 bp in totall The length of the ITS1 was 249 bp and ITS2 was 247 bp respectively. There were a total of 16 mutation loci between sequeneesi [ Conclusion] Determination sequences of Epimedium rDNA ITS could be applied to the evaluation of the au- thentic herb Epimedium asive!l as one of the molecular markers of identification of Chinese medicine Epimedium in molecular level.