NGF及其受体P75在退行性腰椎间盘疾病及腰椎间盘突出症患者椎间盘中表达

Comparison of nerve growth factor and p75 neurotrophinc receptor expression in patients with degenerative disc diseasXe and lumbar disc herniation

目的研究退行性腰椎间盘疾病及椎间盘突出症患者椎间盘中NGF及其受体P75的表达。方法应用免疫组织化学法检测NGF及其受体P75在退行性腰椎间盘疾病及椎间盘突出症患者椎间盘中表达。结果NGF及P75均在椎间盘软骨细胞胞浆中表达,NGF及P75在椎间盘突出症患者椎间盘细胞阳性率分别为25.8%和23.7%,退行性椎间盘疾病患者椎间盘细胞阳性率分别为39.7%和38.7%,退行性椎间盘疾病患者NGF及P75阳性率高于椎间盘突出症患者(P<0.05)。椎间盘退变Pfirrmann分级与椎间盘中NGF及受体P75表达并无明显相关。结论退行性椎间盘疾病椎间盘中NGF及其受体P75较椎间盘突出症表达更高,可能与退行性椎间盘患者腰痛较椎间盘突出症患者腰痛更明显相关。

Objective To investigate the expression of nerve growth factor （NGF） and its receptor, P75 neurotrophin receptor （P75NTR） in disc specimens obtained from patients with lumbar disc herniation （LDH） and degenerative disc disease （DDD）. Methods Human intervertebral disc samples were obtained from patients during surgery. The expression of NGF and P75NTR was detected by immunohistoehemistry method. Results Immune positive staining was observed for both NGF and P75NTR. Cellular staining was predominantly localised intracellularly within the cytoplasm of chondrocyte-like ceils of the nucleus pulposus and annulus fibrous tissue. NGF and P75NTR immunopositivity were 25.8% and 23.7% in disc tissue from LDH patient respectively. For DDD patient, the immunopositivity of NGF and P75NTR were 39.7% and 38.7% respectively. Immunopositivity of NGF and P75NTR were significantly greater in the DDD group than in the LDH group. No statistical correlation between the levels of NGF and P75NTR and the degree of disc degeneration in either DDD group or LDH group was found. Conclusion Our results demonstrate that the greater levels of NGF and P75NTR expression in the DDD group than in the LDH group may contribute more severe low back pain in DDD patients than patients with LDH.