过氧化物酶Ⅰ在正常和骨关节炎膝关节软骨组织中的表达

Expression of PrxⅠin normal and OA cartilage tissues of knee joint

目的 探讨过氧化物酶Ⅰ在正常和骨关节炎膝关节软骨组织中的表达.方法 选取本院2010年10月～2012年10月行单侧膝关节表面置换术的骨关节炎患者39例为观察组,选取本院同期行单侧膝关节表面置换术的非骨关节炎患者26例为对照组,分别提取部分膝关节软骨,采用Western Blot方法检测膝关节软骨组织细胞中过氧化物酶Ⅰ的表达水平.结果 观察组平均积分光度值明显高于对照组,观察组膝关节软骨组织表层、中层、深层的氧化物酶Ⅰ免疫组化染色的平均积分光度值均明显高于对照组,差异有统计学意义（P＜0.05）.对照组膝关节软骨组织表层、中层、深层的氧化物酶Ⅰ免疫组化染色的平均积分光度值呈增加趋势,相互间比较差异均无统计学意义（P＞ 0.05）.观察组膝关节软骨组织表层、中层、深层的氧化物酶Ⅰ免疫组化染色的平均积分光度值呈现明显的增加趋势,相互间比较差异均有统计学意义（P＜0.05）.结论 骨关节炎膝关节软骨组织中过氧化物酶Ⅰ的表达水平显著高于正常膝关节软骨组织中过氧化物酶Ⅰ的表达水平.

Objective To investigate expression of Prx I in normal and OA cartilage tissue of knee joint. Methods A total of 39 OA patients who treated knee arthroplasty were selected in hospital from October 2010 to October 2012 as observation group. 26 non-OA patients who treated knee arthroplasty were selected in hospital at the same period as control group. Part of knee joint cartilage were extracted. Expression of Prx I in cartilage tissue ceils of knee joint was detected by Western blot method. Results Average integral absorbance value in observation group was significantly higher than control group. Average integral absorbance values of knee joint cartilage surface, middle, deep in observa- tion group were significantly higher than control group. Difference was statistically significant （P 〈 0.05）. Average inte- gral absorbance values of knee joint cartilage there was no significant difference among （P 〉 face, middle, deep in observation group showed surface, middle, deep in control group showed increasing trend,while 0,05）, Average integral absorbance values of knee joint cartilage sur- significant increasing trend,while difference was statistically significant （P 〈 0.05）. Conclusion Expression of Prx I in OA cartilage tissue of knee joint is significandy higher than that in nor- real cartilage tissue of knee joint.