数字显示分光光度计中吸光度负值在光度分析中的应用

Application of Negative Absorbance on Digital Spectrophotometer Li Chunchao

本文探讨了应用数字显示分光光度计中吸光度负值的理论根据,其中包括误差分析,标准曲线的绘制和温度对中性滤光片吸光度的影响等。

In this paper the theoretical basis of applying the negative absorbance on a digital spectrophotometer is discussed. The error analysis and the plotting of analytical curve are illustrated. It is shown that the application of negative absorbance will result in wider analytical range, smaller relative error and simpler operation.