阿魏酸对莴苣幼苗根生长发育的影响

Efects of ferulic acid on seedling root growth and development of lettuce

低浓度阿魏酸对莴苣幼根生长发育有一定的促进作用,当浓度为0.05,0.50 mmol/L时,幼根净生长值和有丝分裂指数相对较高,分别为11.47 mm,9.88%和11.09 mm,11.22%.在低浓度下,幼苗根尖细胞有丝分裂前期细胞比率较高,当浓度为0.05,0.50 mmol/L时,根尖细胞有丝分裂前期指数分别为6.57%,7.26%.而高浓度的阿魏酸对莴苣幼根生长发育有抑制作用,当浓度为1.00 mmol/L时,幼根净生长值和有丝分裂指数为1.26 mm,6.41%,有丝分裂前期指数为0.98%.用流式细胞仪检测莴苣根尖分生区细胞周期的变化,结果表明:1.00 mmol/L阿魏酸对莴苣幼苗根尖分生区细胞的细胞周期具有阻滞作用,主要阻滞了S期和G2期,且阻滞效应与阿魏酸的作用时间有关,处理时间越长则阻滞越明显.

The growth of seedling root of lettuce was promoted at a lower concentration of ferulic acid. Pure growth of root and mitotic index were higher under treatment at 0.05, 0.50 mmol/L and their values were 11.47 mm, 9.88% and 11.09 mm, 11.22% respectively. Under the concentration of 0.05, 0.50 mmol/L, the mitosis prophase cell ratio of root tip was higher, aud they were 6.57% and 7.26% respectively. But the growth of seedling root was significantly inhibited with the treatment of 1.00 mmol/L of ferulic acid, the pure growth of root and mitotic index were 1.26 mm and 6.41% respectively and the mitosis prophase cell ratio was 0.98%. Moreover, the cell cycle of root apex under the treatment at 1.00 mmol/L was detected by using flow cytometry. The results indicated that S phase and C2 phase in cell cycle were mainly blocked. The blocked effect was associated with the processing time of ferulic acid, and it became more severe with the increase of processing time.