摘要
[ Objectivel The paper was to study antifungal activity and control mechanism of Bacillus cereus CGMCCA348 against Botrytis cinerea, and further find new methods against the disease. [Method] By quantitative bioassay, in vitro experiment and pot test, the antagonistic protein was extracted with ammonium sul- fate precipitation fractionation to determine the control efficiency. ECso of B. cereus CGMCC4348 was 6. 19 mg/L. Average controlling effect of B. cereus CGM- CC4348 on B. cinerea was 75.8%, equal to the control group which was treated by 50 mg/L pyrimethanil. Bacillus cereus CGMCCA348 had good inhibitory effect against B. cinerea. [ Conclusion] The paper provides scientific basis for green control technology against solanaceous vegetable diseases.
[ Objectivel The paper was to study antifungal activity and control mechanism of Bacillus cereus CGMCCA348 against Botrytis cinerea, and further find new methods against the disease. [Method] By quantitative bioassay, in vitro experiment and pot test, the antagonistic protein was extracted with ammonium sul- fate precipitation fractionation to determine the control efficiency. ECso of B. cereus CGMCC4348 was 6. 19 mg/L. Average controlling effect of B. cereus CGM- CC4348 on B. cinerea was 75.8%, equal to the control group which was treated by 50 mg/L pyrimethanil. Bacillus cereus CGMCCA348 had good inhibitory effect against B. cinerea. [ Conclusion] The paper provides scientific basis for green control technology against solanaceous vegetable diseases.
基金
Supported by Science and Technology Support(Agriculture) Program of Jiangsu Province(BE2012373)&Science and Technology Support Program of Jiangsu Province(BE2013442)
