氨基硅烷化磁性纳米微球的制备及性能研究

Preparation of Aminosilane Modified Magnetic Nanomicrospheres and Its Performance Study

采用化学共沉淀法合成磁性Fe3O4纳米粒子（Magnetic Nanoparticles，MNP），先后用正硅酸乙酯（TEOS）和3-氨丙基三乙氧基硅烷（APTEs）对MNP进行表面功能修饰，成功制备了硅烷化磁性纳米粒子（SMNP）和氨基磁性纳米微球（AMNP）。通过透射电镜（TEM）、傅立叶红外变换光谱仪（FTIR）、X-射线衍射仪（XRD）、超导量子干涉仪（SQUID）和热重分析仪（TGA）对合成粒子进行形貌、结构、磁性能和化学组成进行表征。测试结果表明，合成材料平均粒径约为7～10nm，结晶性能良好；3种粒子均具有良好的超顺磁性，MNP、SMNP和AMNP的饱和磁化强度值分别为47．9、38．0和19．5emu／g；氨基含量为5．7％。利用AMNP对牛血清白蛋白（BSA）开展酶的固定化研究，最大吸附量为41mg／g。

Magnetic Fe304 nanoparticles （MNP） was firstly synthesized by traditional chemical coprecipi-tation method. The synthesized magnetic Fe304 nanoparticles （MNP） was further modified successively with tetraethyl orthosilicate （TEOS） and 3-aminopropyl triethylenesilane （APTES）. Silanization magnetic nanoparticles （SMNP） and amino magnetic nanomicrosphere （AMNP） were abtained successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its deriva-tives were characterized using transmission electron microscopy（TEM）, FT-IR analysis, X-ray diffrac- tion, superconducting quantum interference device（SQUID） and thermogravimetric analyses （TGA）. The characterization results showed the average diameters of the synthetic materials were about 7~10 nm. All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47. 9, 33.0 and 19. 5 emu/g, respectively. The amino content was 5.7%. AMNP was used to immobilize bovine serum albumin （BSA）, and the max-imum adsorption capacity of the protein was 41 mg/g.