摘要
采用化学共沉淀法合成磁性Fe3O4纳米粒子(Magnetic Nanoparticles,MNP),先后用正硅酸乙酯(TEOS)和3-氨丙基三乙氧基硅烷(APTEs)对MNP进行表面功能修饰,成功制备了硅烷化磁性纳米粒子(SMNP)和氨基磁性纳米微球(AMNP)。通过透射电镜(TEM)、傅立叶红外变换光谱仪(FTIR)、X-射线衍射仪(XRD)、超导量子干涉仪(SQUID)和热重分析仪(TGA)对合成粒子进行形貌、结构、磁性能和化学组成进行表征。测试结果表明,合成材料平均粒径约为7~10nm,结晶性能良好;3种粒子均具有良好的超顺磁性,MNP、SMNP和AMNP的饱和磁化强度值分别为47.9、38.0和19.5emu/g;氨基含量为5.7%。利用AMNP对牛血清白蛋白(BSA)开展酶的固定化研究,最大吸附量为41mg/g。
Magnetic Fe304 nanoparticles (MNP) was firstly synthesized by traditional chemical coprecipi-tation method. The synthesized magnetic Fe304 nanoparticles (MNP) was further modified successively with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were abtained successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its deriva-tives were characterized using transmission electron microscopy(TEM), FT-IR analysis, X-ray diffrac- tion, superconducting quantum interference device(SQUID) and thermogravimetric analyses (TGA). The characterization results showed the average diameters of the synthetic materials were about 7~10 nm. All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47. 9, 33.0 and 19. 5 emu/g, respectively. The amino content was 5.7%. AMNP was used to immobilize bovine serum albumin (BSA), and the max-imum adsorption capacity of the protein was 41 mg/g.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2013年第11期71-76,共6页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目(2011AA090703)
国家自然科学基金项目(31200642)
青岛市科技计划项目(12-1-4-12-(2)-jch)
中国水产科学研究院基本科研业务费(2013A1002)资助