组织蛋白酶L及其组织抑制剂在卵巢恶性肿瘤组织中的表达及其意义

EXPRESSION OF CATHEPSIN B AND CYSTATIN C IN OVARIAN CANCER

目的:探讨组织蛋白酶L(Cathepsin L,CL)及其组织抑制剂C(Cystatin C,CC)在卵巢恶性肿瘤中的表达及与卵巢恶性肿瘤临床病理特征的关系。方法:应用RT-PCR方法检测47例卵巢恶性肿瘤、21例良性卵巢肿瘤、20例正常卵巢组织中CL及CC的表达情况,行阳性率及半定量的比较并将其结果与临床病理学资料进行分析。结果:CL在卵巢恶性肿瘤中的表达水平明显高于良性肿瘤和正常卵巢组织中(P<0.05)。CL半定量表达与临床分期、病理分级、淋巴结转移有关(P<0.05)。CC在卵巢恶性肿瘤中的表达水平明显高于良性肿瘤和正常卵巢组织(P<0.01)。CC半定量表达在病理分级、肝转移、大网膜转移、腹水方面比较,差异有统计学意义(P<0.05)。结论:CL、CC在卵巢恶性肿瘤中表达增高,可能与卵巢恶性肿瘤转移相关。

Objective. The aim of this study was to investigate the expression of cathepsin L and cystatin C in ovarian cancer and clinicopathologic characteristics in patients with ovarian cancer. Methods： Tissue speci- mens were divided into three groups, normal ovary, benign neoplasm, and ovarian cancer. RT-PCR was performed to detected the expression of cathepsin L and cystatin C mRNA. Results.. The semi-quantity of cathepsin L mRNA was significantly higher in ovarian malignant tumor than that in benign neoplasm tissue and normal ovarian tissue （ P 〈0.05）. The mRNA expression of cathepsin L was correlated with clinical stages, differentiation degree of cancer cells and lymph node metastasis （ P 〈0.05）. The semi-quantity of cystatin C mRNA was significantly higher in ovarian malignant tumor than that in benign neoplasm tissue and normal ovarian tissue （P 〈0.01）. The mRNA expression of cystatin C was correlated with differen- tiation degree of cancer cells and liver metastasis and omentum majus metastasis （ P 〈0.05）. Conclusion： The results provide convincing evidence that cathepsin L and cystatin C may contrihute to the mechanisms of invasion and metastatsis of ovarian cancer.