地黄内生真菌中一个新的二苯醚类代谢产物(英文)

A new diphenyl ether from the endophytic fungus Verticillium sp.isolated from Rehmannia glutinosa

目的:研究地黄内生真菌的代谢产物。方法:采柱色谱方法进行分离纯化,并根据化合物的理化性质和波谱数据鉴定其结构。结果:从地黄内生真菌代谢产物中分离并鉴定了10个化合物:2,4-dihydroxy-2’,6-diacetoxy-3’-methoxy-5’-methyldiphenyl ether(1),paecilospirone(2),α-axetylorcino(3),2-methoxy-1,8-dimethyl-xanthen-9-one(4),4-hydroxy-α-lapachone(5),enalin A(6),2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran(7),p-hydroxyethyl-phenol(8),2,4-dihydroxy-3,5,6-trimethyl-methylbenzoate(9),3-isopropenyl-(z)-monomethyl maleate(10)。结论:化合物1为新的二苯醚类化合物,其显示了一定的细胞毒和抗真菌活性。

AIM： To investigate the chemical constituents of the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa. METHODS： The compounds were isolated and purified by repeated column chromatography, and their structures were determined on the basis of physicochernical properties and spectral analysis. Their cytotoxic and antifungal activities were evaluated. RESULTS： Ten compounds were obtained and their structures were identified as 2, 4-dihydroxy-2＇, 6-diacetoxy-3＇-methoxy-5＇-me- thyl-diphenyl ether （1）, paecilospirone （2）, a-acetylorcinol （3）, 2-methoxy-1,8-dimethyl-xanthen-9-one （4）, 4-hydroxy-a-lapachone （5）, enalin A （6）, 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran （7）, 4-hydroxyethyl-phenol （8）, 2,4-dihydroxy-3,5,6-trimethyl- methylbenzoate （9）, and 3-isopropenyl-（Z）-monomethyl maleate （10）. CONCLUSIONS： Compound 1 is a new diphenyl ether, and showed cytotoxic activity against HL-60 cells （IC50 2.24 μg.mL^-1）, and antifungal activities against Candida albicans （MIC 8μg.mL^-1） and Aspergillusfumigatus （MIC 16μg.mL^-1）.