α-三噻吩和抗生素对松材线虫运动性的影响

Inhibitory Effects of α-Terthienyl and Antibiotics on Dispersal Ability of Bursaphelenchus xylophilus

松萎蔫病是由松材线虫与细菌诱导的复合侵染学说为抗生素应用于松萎蔫病防治提供了理论依据,本试验主要研究杀线剂α-三噻吩(α-T)和抗生素对松材线虫运动性的影响。在30种抗生素中筛选了对松材线虫携带的10个致病细菌菌株具有抑菌活性的抗生素,研究了α-T对松材线虫携带的致病细菌的抑制作用,并以黑松枝为材料,研究了在不同α-T、抗生素以及二者1∶1混合液处理条件下,松材线虫在黑松枝条中运动性变化。结果筛选出环丙沙星和头孢曲松钠,试验证明其对松材线虫携带的致病细菌具有强抑菌活性,α-T对松材线虫携带的致病细菌无抑制作用,α-T、抗生素及二者混合处理对松材线虫运动性都具有显著抑制作用,在0.11μg/g时,α-T处理对松材线虫运动性抑制率为42.3%,抗生素处理抑制率为23.0%,二者1∶1混合使用抑制率为60.3%,说明抗生素与α-T之间在一定质量浓度范围内可能存在增效作用。

Pine wilt disease is a disease complex resulted from infection by both pine wood nematode （PWN）,Bursaphelenchus xylophilus,and the pathogenic bacteria they carry.This theory has founded the base for using antibiotics to control pine wilt disease.Two antibiotics were screened from 30 antibiotics by testing their antibiotic activity to 10 pathogenic bacteria the nematode carry.The effects of α-T on the pathogenic bacteria,the inhibition of antibiotics,α-Terthienyl （α-T） and their mixture （antibiotics：α-T =1 ∶ 1） on dispersal ability of PWN were examined.The results showed that Ceftriaxone Sodium and Ciprofloxacin could significantly inhibit production of pathogenic bacteria,but α-T didn＇ t affect the bacteria,and that both the antibiotics and α-T could inhibit dispersal ability of PWN significantly.The inhibitory values of α-T,the antibiotics and their mixture （1 ∶ 1） on dispersal ability of PWN at 0.11 μg/g were：42.3％,23.0％ and 60.3％respectively,which indicates that synergism may exist between the antibiotics and α-T at certain levels.