柑橘皮中抑菌活性成分的提取

The Research on Extraction of Antimicrobial Components from Citrus Peels

文章用溶剂提取法从柑橘皮中提取抑菌活性成分。用滤纸片法和最低抑菌浓度法(MIC)判断提取物对大肠杆菌的抑菌效果,确定最佳提取条件。结果表明:乙醇浓度90%、提取温度50℃、提取时间4 h时的提取物对供试菌的抑菌活性显著。短时间(10 min)热处理(100℃)对提取物的抑菌效果无影响。

The antimicrobial ingredients were extracted from citrus peel by solvent. Filter paper method and minimum inhibitory concentration method （MIC） were adopted to detect the antibacterial activity of extracts against E.coli, and the optimum extraction conditions were determined. The results show that： the ethanol concentration was 90 %, extraction temperature was 50 ~C, extraction time was 4 h. The extract showed antibacterial activity on the tested strains significantly. There is no effect on the antibacterial effects of the extract which was suffered short time heating treatment.