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同时携带TEM-1、ADC-57、DHA-1、OXA-23 4种β-内酰胺酶基因的泛耐药鲍曼不动杆菌的发现 被引量：3

Detection of pandrug-resistant Acinetobacter baumannii carrying TEM-1,ADC-57,DHA-1 and OXA-23 β-lactamases encoding genes simultaneously
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摘要 目的探讨临床分离泛耐药鲍曼不动杆菌(PDR-ABA)β-内酰胺酶编码基因的存在状况。方法使用PCR法对20株PDR-ABA菌进行14种A类、10种B类β-内酰胺酶编码基因(金属β-内酰胺酶)、2种C类和8种D类β-内酰胺酶编码基因(合计34种β-内酰胺酶基因)的基因检测,并使用PCR法对插入序列ISAba1与β-内酰胺酶bla OXA-23基因(ISAba1-OXA-23)、插入序列ISAba1与β-内酰胺酶ADC-57基因(ISAba1-ADC-57)的进行连锁检测。对PCR阳性产物使用全自动荧光法进行测序。结果 20株PDR-ABA均检出bla TEM-1、bla ADC-57、bla DHA-1和bla OXA-23的4种β-内酰胺酶基因,且ISAba1-OXA-23和ISAba1-ADC-57的连锁检测均为阳性。结论本组菌株耐全部β-内酰胺类药物原因可能与其产TEM-1、ADC-57、DHA-1和OXA-23等四种β-内酰胺酶相关,且ADC-57和OXA-23这2种β-内酰胺酶基因的高表达对耐药贡献率更高。 Abstract Objective To investigate the distribution and variety of β-1actamase genes in a group of clinical isolated pandrug resistant of Acinetobacter baumannii （PDR-ABA）. Methods 14 kinds of class A ^-lactamase, 10 kinds of class B β-1actamase （AmpC β-1actamase）, 2 kinds of class C β-1actamase and 8 class kinds of D β-1actamase genes were tested by PCR.We also linkage analyzed the insertion sequence（IS） ISAbal with blaoxg.23 gene （ISAba1- OXA-23） and the IS ISAbal with b[aADC.57 gene （ISAba1-ADC-57） by PCR in the 20 strains. Then, we used fully automated fluorescence method for sequencing of PCR positive products. Results In 20 strains of PDR-ABA, we also detected β-1actamase genes of the blaTEM.1, blaAoc.57, blaoHA.1 and blaoxA.23. The linkage analysis of ISAbal- OXA-23 and 1SAba1-ADC-57 was positive. Conclusion All the 20 strains of Acinetobacter baumannii with the β-1actamases genes of blaTEM.1, blaADC.57, blaDHA.1 and blaoxA.23 maybe lead to resistant to all the β-1actams, and β- lactamase genes of blaADC.57 and blaoxA_23 highly expressed has higher contribution to the rate of resistance.
作者 蔡培泉 李朋玲 王春新
机构地区 南京医科大学附属无锡市人民医院呼吸内科 南京医科大学附属无锡市人民医院检验科
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2013年第11期I0004-I0008,共5页 Chinese Journal of Antibiotics
关键词 泛耐药鲍曼不动杆菌 Β-内酰胺类 耐药基因 Β-内酰胺酶 插入序列 Pan-resistant Acinetobacter baumannii baumactams Resistance gene β-lactamases Insertion sequence
分类号 R378 [医药卫生—病原生物学]
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参考文献10

  • 1Munoz-Price L S, Weinstein R A. Acinetobacter infection[J]. NEnglJMed, 2008, 358(12): 1271-1281.
  • 2Vashist J, Tiwari V, Das R, et al. Analysis of penicillin- binding proteins(PBPs) in carbapenem resistant Aeinetobaeter baumannii[J]. Indian J Med Res, 2011, 133(3): 332-338.
  • 3Doi Y, Husain S, Potoski BA, et al. Extensively drug- resistant Acinetobacter baumannii[J]. Emerg Infect Dis, 2009, 15(6): 980-982.
  • 4王辉,倪语星,陈民钧,胡必杰,王明贵,陈佰义,胡继红,胡云建.新型甘氨酰环素类抗菌药物替加环素的体外药敏试验操作规程[J].中华检验医学杂志,2009,32(11):1208-1213. 被引量：65
  • 5Heritier C, Poirel L, Nordmann P. Cephalosporinase over-expression resulting from insertion of ISAbal in Acinetobacter baumannii[J].Clin Microbiol Infect, 2006,12(2): 123-130.
  • 6Corvec S, Poirel L, Naas T, et al. Genetics and Expression of the carbapenem-hydrolyzing oxacillinase gene blaoxA.23 in Acinetobacter baumannii[J]. Antimicrob Agents Chemother, 2007, 51(4): 1530-1533.
  • 7Jacoby G A, Munoz-Price L S. The new 13-1actamases[J]. N Engl JMed, 2008, 352(4): 380-391.
  • 8Perez F, Hujer A M, Hujer K M, et al. Global challenge of multidrug-resistant Acinetobacter baumannii[J]. Antimicrob Agents Chemother, 2007, 51(10): 3471-3484.
  • 9Wong W T, Au H W, Yap H K, et al. Structural studies of the mechanism for biosensing antibiotics in a fluoresceinlabeled 13-1actamase [J]. BMC Str Bio, 2011, 11 (15): 1-8.
  • 10Queenan A M, Bush K. Carbapenemases: the Versatile 13-Lactamases[J]. Clin Microbiol Rev, 2007, 20(3): 440-457.

二级参考文献23

  • 1Petersen PJ, Jacobus NV, Weiss WJ, et al. In vitro and in vivo antibacterial activities of a novel glycylcycline, the 9-t-butylglycylamido derivative of minocycline (GAR-936). Antimicrob Agents Chemother, 1999, 43:738-744.
  • 2Bradford P. Tigecycline: a first in class glycylcycline. Clin Micro Newsl, 2004,26:163-168.
  • 3Felmingham D. Tigecycline-the first glycylcycline to undergo clinical development: an overview of in vitro activity compared to tetracycline. J Chemother, 2005,17 Suppl 1:5-11.
  • 4Chopra I, Hawkey PM, Hinton M. Tetracyclines, molecular and clinical aspects. J Antimicrob Chemother,1992 ,29 :245-277.
  • 5Schnappinger D, Hillen W. Tetraeyclines: antibiotic action, uptake, and resistance mechanisms. Arch Mierobiol, 1996, 165:359-369.
  • 6Bauer G, Berens C, Projan SJ, et al. Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drugdirected Fe^2 + cleavage of 16S rRNA. J Antimicrob Chemother, 2004, 53:592-599.
  • 7Olson MW, Ruzin A, Feyfant E, et al. Functional, biophysical, and structural bases for antibacterial activity of tigecycline. Antimicrob Agents Chemother, 2006,50 : 2156 -2166.
  • 8Keeney D, Ruzin A, Bradford PA. RamA, a transcriptional regulator, and AcrAB, an RND-type efflux pump, are associated with decreased susceptibility to tigecycline in Enterobacter cloacae. Mierob Drug Resist, 2007,13:1-6.
  • 9Ruzin A, Visalli MA, Keeney D, et al. Influence of transcriptional activator RamA on expression of multidrug efflux pump AcrAB and tigecycline susceptibility in Klebsiella pneumoniae. Antimicrob Agents Chemother,2005,49 : 1017-1022.
  • 10Visalli MA, Murphy E, Projan SJ, et al. AcrAB multidrug efflux pump is associated with reduced levels of susceptibility to tigecycline ( GAR- 936) in Proteus mirabilis. Antimicrob Agents Chemother. 2003.47,665-669.

共引文献64

同被引文献31

  • 1王辉,孙宏莉,廖康,陈冬梅,王清涛,王飞燕,陈民钧,朱元珏.北京和广州地区四家医院不动杆菌碳青霉烯酶基因型研究[J].中华检验医学杂志,2005,28(6):636-641. 被引量：121
  • 2郭萍,王辉,陈端,杨启文,徐英春,陈民钧.OXA-51样D类碳青霉烯酶在鲍曼不动杆菌中广泛分布[J].中华检验医学杂志,2007,30(5):505-509. 被引量：19
  • 3Woodforct N, Ellington MJ, Coelho JM, et aT. Multiplex PCR fi:r genes encoding prev',dent OXA earbapenemases in Acinetobacter spp [ J]. lnt J Antimierob Agents, 2006, 27(4) : 351-353.
  • 4I-Iiggins PG, Dammhayn C, Hackel M, et al. Global spread of car- bapenem-resistant Acinetobacter baumannii [ J ]. J Antimicrob Che- mother, 2010, 65(2): 233-238.
  • 5Ayse NS, Meral B, Zeynep G, et al. The first report on the out- break of OXA-24/40-like carbapenemase-producing Acinetobacter baumannii in Turkey[J]. Japan J Infect Dis, 2013, 66(5) : 439- 442.
  • 6Wang H, Guo P, Sun HL, et al. Molecular epidemiology of clinical isolates of Carbapenem-resistant Acinetobacter spp. from Chinese hos- pitals[J]. Antimicrob Agents Chemother, 2007, 51(11): 4022-4028.
  • 7Joshi Acosta, Maria Merino, Esther Viedma, et al. Muhidrug-re- sistant Acinetobacter baumannii Harboring OXA-24 Carbapenemase, Spain[J]. Emerg Infect Dis, 2011, 17(6) : 1064-1067.
  • 8Mariana Castanheira, Audrey Wanger, Mark Kruzel, et al. Emer- gence and clonal dissemination of OXA-24- and OXA-58-pmducing Acinetobacter baumannii strains in Houston, Texas: Report from the SENTRY antimicrobial surveillance program [ J ]. J Clin Microbiol, 2008, 46(9): 3179-3180.
  • 9Maria Merino, Joshi Acosta, Margarita Poza, et al. OXA-24 Car- bapenemase gene flanked by XerC/XerD-like recombination sites in different plasmids from different Acinetobacter species isolated during a nosocomial outbreak[ J]. Antimicrob Agents & Hemother, 2010, 54(6): 2724-2727.
  • 10Chopra T, Marchaim D, Awali RA, et al. Epidemiology ofbloodstream infections caused by Acinetobacter baumannii and impact of drug resistance to both carbapenems and ampicillin- sulbactam on clinical outcomes [ J ]. Antimicrob Agents Che- mother, 2013, 57(12): 6270-6275.

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中国抗生素杂志
2013年 第11期

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