川硬皮肿腿蜂毒液蛋白的分离纯化

Isolation and Purification of the Venom Proteins in Sclerodermus sichuanensis

为揭示川硬皮肿腿蜂寄生对寄主黄粉虫蛹的麻痹机制,通过自然寄生和Sephadex G75层析等方法研究寄生过程中毒液的作用,并对毒液中一种麻痹蛋白进行分离纯化。结果表明:川硬皮肿腿蜂毒液具有麻痹作用,且毒液中存在一种相对分子质量约为51.17 ku的麻痹蛋白。寄主被麻痹的程度与毒液注入量呈正相关,恢复活动情况与毒液注入量呈负相关;当人工注射毒液浓度为0.01 VRE时,黄粉虫蛹表现出可逆的轻微麻痹,但羽化蜕皮被抑制;当人工注射浓度提高到0.2 VRE时,黄粉虫蛹表现出不可逆的完全僵化。另外,川硬皮肿腿蜂毒液中存在6种大分子毒液蛋白,其中相对分子量约为51.17 ku的毒液蛋白vpr 4具有麻痹活性,且对寄主黄粉虫蛹的麻痹活性程度表现不平衡。

To explore the parasitic regulatory mechanism of parasitism by Sclerodermus sichuanensis on the host,Tenebrio molitor,the venom injection and Sephadex G75 chromatography were conducted to investigate the effects of venom on the host pupa in the parasitic process. Additionally,a parasitic venom protein was isolated. The results showed that the venom had paralytic effects,and an approximate 51. 17 ku paralytic venom protein was isolated and characterized. The paralytic degree had a positive correlation with the concentration of venom injection,and the number of recovered pupa had a negative correlation with the concentration. T. molitor pupa was in slight and reversible paralysis and its exuviate was inhibited when injected with 0. 01 VRE（ venom reservoir equivalent） venom artificially. The parplysis was nonreversible and complete when the concentration of injected venom rose to 0. 2 VRE. Six kinds of macromolecular venom proteins were found in the venom,and among them an approximate 51. 17 ku venom protein vpr 4 had paralytic activity,but the paralytic activity was unbalance.